Real-time full-spectral imaging and affinity measurements from 50 microfluidic channels using nanohole surface plasmon resonance.
Real-time full-spectral imaging and affinity measurements from 50 microfluidic channels using nanohole surface plasmon resonance.
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DOI：
10.1039/c2lc40455a
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发表时间：
2012-10-21
期刊：
影响因子：
6.1
通讯作者：
Oh SH
中科院分区：
文献类型：
--
作者：
Lee SH;Lindquist NC;Wittenberg NJ;Jordan LR;Oh SH
随着高通量蛋白质组学和系统生物学的最新进展，对能够以高通量方式精确定量广泛的受体-配体结合动力学的新仪器的需求不断增长。在这里，我们展示了一个表面等离子体共振（SPR）成像光谱仪能够同时从50个平行的微流体通道提取结合动力学和亲和力。该仪器利用大面积（~ cm 2）金属纳米孔阵列作为SPR传感基底，并结合宽带光源，高分辨率成像光谱仪和低噪声CCD相机，以真实的时间从每个通道提取光谱信息，折射率分辨率为7.7 × 10−6。为了证明我们的仪器用于定量广泛的生物分子相互作用的实用性，每个平行的微流体通道涂覆有含有神经节苷脂（GM 1）受体的仿生支持的脂质膜。然后在单个实验中从50个通道测量霍乱毒素B（CTX-B）与GM 1的结合动力学。通过将高度并行的微流体装置与大面积周期性纳米孔阵列芯片相结合，我们的SPR成像光谱仪系统能够实现高通量、无标记、实时SPR生物传感，并且其与纳米孔阵列相结合的全光谱成像能力可以实现SPR成像与并发表面增强拉曼光谱的集成。
With recent advances in high-throughput proteomics and systems biology, there is a growing demand for new instruments that can precisely quantify a wide range of receptor-ligand binding kinetics in a high-throughput fashion. Here we demonstrate a surface plasmon resonance (SPR) imaging spectroscopy instrument capable of extracting binding kinetics and affinities from 50 parallel microfluidic channels simultaneously. The instrument utilizes large-area (~cm2) metallic nanohole arrays as SPR sensing substrates and combines a broadband light source, a high-resolution imaging spectrometer and a low-noise CCD camera to extract spectral information from every channel in real time with a refractive index resolution of 7.7 × 10−6. To demonstrate the utility of our instrument for quantifying a wide range of biomolecular interactions, each parallel microfluidic channel is coated with a biomimetic supported lipid membrane containing ganglioside (GM1) receptors. The binding kinetics of cholera toxin b (CTX-b) to GM1 are then measured in a single experiment from 50 channels. By combining the highly parallel microfluidic device with large-area periodic nanohole array chips, our SPR imaging spectrometer system enables high-throughput, label-free, real-time SPR biosensing, and its full-spectral imaging capability combined with nanohole arrays could enable integration of SPR imaging with concurrent surface-enhanced Raman spectroscopy.
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影响因子：
7.4
作者：
Jonsson, Magnus P.;Dahlin, Andreas B.;Hook, Fredrik
通讯作者：
Hook, Fredrik
影响因子：
8.4
作者：
Im H;Wittenberg NJ;Lesuffleur A;Lindquist NC;Oh SH
通讯作者：
Oh SH
影响因子：
64.8
作者：
Ebbesen, TW;Lezec, HJ;Wolff, PA
通讯作者：
Wolff, PA
DOI：
10.1088/0034-4885/75/3/036501
发表时间：
2012-03
期刊：
Reports on progress in physics. Physical Society (Great Britain)
影响因子：
--
作者：
Lindquist NC;Nagpal P;McPeak KM;Norris DJ;Oh SH
通讯作者：
Oh SH
影响因子：
3.9
作者：
Brolo, AG;Gordon, R;Kavanagh, KL
通讯作者：
Kavanagh, KL