Exploring the use of APTS as a fluorescent reporter dye for continuous glucose sensing.
Exploring the use of APTS as a fluorescent reporter dye for continuous glucose sensing.
复制标题
探索使用 APTS 作为连续葡萄糖传感的荧光报告染料。
DOI：
--
复制
发表时间：
2009
影响因子：
3.2
通讯作者：
B. Singaram
中科院分区：
文献类型：
--
作者：
Zachary Sharrett;Soya Gamsey;Lacie C. Hirayama;Boaz Vilozny;J. Suri;R. Wessling;B. Singaram
合成了阴离子荧光染料氨基芘三磺酸 (APTS)，并将其用于基于溶液的双组分葡萄糖传感系统，该系统包含该染料和附加硼酸的紫精。染料的荧光在紫精存在下被猝灭，并且在添加葡萄糖后荧光恢复。这种荧光团的一个重要特征是它可以通过胺基团与聚合物共价键合，而不会对光学性能产生显着影响。合成了两种用可聚合基团官能化的 APTS 衍生物，并将其固定在基于甲基丙烯酸羟乙酯 (HEMA) 的水凝胶中。后者用于连续监测血糖。评估了水凝胶的荧光信号调制、信号稳定性、可逆性、重现性和 pH 敏感性。本文所述的APTS染料在溶液中和固定在水凝胶中时对生理范围内的pH变化不敏感。当 APTS 与添加硼酸的紫精结合使用来检测葡萄糖时，由于硼酸的存在，系统表现出一定的 pH 敏感性。
The anionic fluorescent dye, aminopyrene trisulfonic acid (APTS), was synthesized and used in a solution-based two-component glucose-sensing system comprising the dye and a boronic acid-appended viologen. The fluorescence of the dye was quenched in the presence of the viologen and the fluorescence restored upon glucose addition. An important feature of this fluorophore is that it can be covalently bonded to a polymer through the amine group without a significant effect on optical properties. Two APTS derivatives, functionalized with polymerizable groups, were synthesized and immobilized in hydroxyethyl methacrylate (HEMA)-based hydrogels. The latter were used to continuously monitor glucose. The fluorescence signal modulation, signal stability, reversibility, reproducibility, and pH sensitivity of the hydrogels were evaluated. The APTS dyes described herein are insensitive to pH changes within the physiological range, both in solution and when immobilized in a hydrogel. When APTS is used in conjunction with boronic acid-appended viologens to sense glucose, the system displays some pH sensitivity because of the presence of the boronic acid.
登录
查看更多内容
影响因子：
2.9
作者：
GIULIANO, KA;GILLIES, RJ
通讯作者：
GILLIES, RJ
影响因子：
1.8
作者：
DiCesare, N;Lakowicz, JR
通讯作者：
Lakowicz, JR
影响因子：
4.9
作者：
Springsteen, G;Wang, BH
通讯作者：
Wang, BH
影响因子：
3.9
作者：
DiCesare, N;Pinto, MR;Lakowicz, JR
通讯作者：
Lakowicz, JR