The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region.
The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region.
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粗糙脉孢菌高表达的蛋氨酸合酶基因受其近端异染色质区域正向调控
DOI：
10.1093/nar/gku261
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发表时间：
2014-06
影响因子：
14.9
通讯作者：
He Q
中科院分区：
文献类型：
--
作者：
Yang S;Li W;Qi S;Gai K;Chen Y;Suo J;Cao Y;He Y;Wang Y;He Q
在粗糙脉孢菌中，甲硫氨酸合成酶基因met-8在甲硫氨酸合成中起关键作用。在这项研究中，我们发现MET-8蛋白水平在几个异染色质形成缺陷的突变体中受到损害。生物信息学分析显示，一个50 kb的AT丰富的区域毗邻的met-8启动子。ChIP分析证实，三甲基化H3K9在该区域富集，表明异染色质可能在met-8的上游形成。在H3K9R突变株中，met-8的输出显著降低，与我们在异染色质形成缺陷的突变株中观察到的相似。此外，在正常异染色质环境的情况下，异位表达的Met-8在His-3位点的生产是低效的，而异位表达的Met-8下游的其他两个异染色质结构域是有效的。此外，我们的数据表明，mig-6的表达也控制了上游4.2 kb AT丰富的区域类似的met-8基因，我们证明，AT丰富的区域相邻的met-8或mig-6所需的峰值表达。我们的研究表明，met-8和mig-6可能代表了一种新的基因类型，其表达依赖于附近异染色质区域的正确形成。
In Neurospora crassa, the methionine synthase gene met-8 plays a key role in methionine synthesis. In this study, we found that MET-8 protein levels were compromised in several mutants defective in proper heterochromatin formation. Bioinformatics analysis revealed a 50-kb AT-rich region adjacent to the met-8 promoter. ChIP assays confirmed that trimethylated H3K9 was enriched in this region, indicating that heterochromatin may form upstream of met-8. In an H3K9R mutant strain, the output of met-8 was dramatically reduced, similar to what we observed in mutant strains that had defective heterochromatin formation. Furthermore, the production of ectopically expressed met-8 at the his-3 locus in the absence of a normal heterochromatin environment was inefficient, whereas ectopic expression of met-8 downstream of two other heterochromatin domains was efficient. In addition, our data show that the expression of mig-6 was also controlled by an upstream 4.2-kb AT-rich region similar to that of the met-8 gene, and we demonstrate that the AT-rich regions adjacent to met-8 or mig-6 are required for their peak expression. Our study indicates that met-8 and mig-6 may represent a novel type of gene, whose expression relies on the proper formation of a nearby heterochromatin region.
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DOI：
10.1073/pnas.1000328107
发表时间：
2010-05-04
影响因子：
11.1
作者：
Lewis, Zachary A.;Adhvaryu, Keyur K.;Selker, Eric U.
通讯作者：
Selker, Eric U.
影响因子：
16
作者：
Freitag, M;Hickey, PC;Selker, EU
通讯作者：
Selker, EU
影响因子：
2.3
作者：
Higa LA;Zhang H
通讯作者：
Zhang H
影响因子：
7.7
作者：
Fourel, G;Boscheron, C;Gilson, É
通讯作者：
Gilson, É
影响因子：
13.8
作者：
Jackson, Sarah;Xiong, Yue
通讯作者：
Xiong, Yue