Factors that influence helical preferences for singly charged gas-phase peptide ions: the effects of multiple potential charge-carrying sites.
Factors that influence helical preferences for singly charged gas-phase peptide ions: the effects of multiple potential charge-carrying sites.
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DOI：
10.1021/jp9105103
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发表时间：
2010-01-21
期刊：
影响因子：
--
通讯作者：
Russell DH
中科院分区：
文献类型：
--
作者：
McLean JR;McLean JA;Wu Z;Becker C;Pérez LM;Pace CN;Scholtz JM;Russell DH
离子淌度-质谱法用于研究一系列模型肽 [M + H]+ 离子的结构，以更好地了解内在特性如何影响低介电环境中的结构。针对先前在溶液中研究的一系列模型肽，检查了肽长度、氨基酸序列和组成对气相结构的影响。报告了 Ac-(AAAKA)nY-NH2 (n = 3 – 6) 和 Ac-Y(AEAAKA)nF-NH2 (n = 2 – 5) 的 [M + H]+ 离子的碰撞截面，并与使用分子建模技术获得的候选结构相关联。 AAKAA 肽系列的 [M + H]+ 离子均表现出与部分螺旋结构相关的单一主导离子淌度到达时间分布 (ATD)，而 AEAAKA 离子系列的 [M + H]+ 离子由与电荷溶剂化小球相关的 ATD 组成（即电荷通过极性肽官能团协调或溶剂化）。这些数据提出了许多关于决定气相肽离子结构的内在特性（氨基酸序列和组成以及电荷位置）的问题，这可能反映了低介电环境中肽离子结构的趋势，例如跨膜片段。
Ion mobility-mass spectrometry is used to investigate the structure(s) of a series of model peptide [M + H]+ ions to better understand how intrinsic properties affect structure in low dielectric environments. The influence of peptide length, amino acid sequence and composition on gas-phase structure is examined for a series of model peptides that have been previously studied in solution. Collision cross-sections for the [M + H]+ ions of Ac-(AAKAA)nY-NH2 (n = 3 – 6) and Ac-Y(AEAAKA)nF-NH2 (n = 2 – 5) are reported and correlated with candidate structures generated obtained using molecular modeling techniques. The [M + H]+ ions of the AAKAA peptide series each exhibit a single, dominant ion mobility arrival time distribution (ATD) which correlates to partial helical structures, whereas the [M + H]+ ions of the AEAAKA ion series are composed of ATDs which correlate to charge-solvated globules (i.e. the charge is coordinated or solvated by polar peptide functional groups). These data raise numerous questions concerning intrinsic properties (amino acid sequence and composition as well as charge location) that dictate gas-phase peptide ion structure, which may reflect trends for peptide ion structure in low dielectric environments, such as transmembrane segments.
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影响因子：
3.3
作者：
Koeniger, SL;Merenbloom, SI;Clemmer, DE
通讯作者：
Clemmer, DE
影响因子：
3.3
作者：
Kohtani, M;Jarrold, MF;O'Hair, RAJ
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影响因子：
5.6
作者：
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影响因子：
2.9
作者：
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通讯作者：
HONIG, BH
影响因子：
3.3
作者：
Hilderbrand, AE;Clemmer, DE
通讯作者：
Clemmer, DE