Blood metallothionein transcript as a biomarker for metal sensitivity: low blood metallothionein transcripts in arsenicosis patients from Guizhou, China.
Blood metallothionein transcript as a biomarker for metal sensitivity: low blood metallothionein transcripts in arsenicosis patients from Guizhou, China.
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DOI：
10.1289/ehp.10035
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发表时间：
2007-07
影响因子：
10.4
通讯作者：
Waalkes MP
中科院分区：
文献类型：
--
作者：
Liu J;Cheng ML;Yang Q;Shan KR;Shen J;Zhou Y;Zhang X;Dill AL;Waalkes MP
由于金属硫蛋白(MT)是一种金属结合蛋白，可以抵抗金属中毒，因此它可以作为个体对金属毒性敏感性的生物标志物。我们评估了血液中MT转录本作为组织MT水平的反映，并研究了MT在环境暴露人群中砷中毒中的潜在作用。用锌或非金属MT诱导剂处理大鼠4d，采集血液和组织，用实时定量逆转录聚合酶链式反应检测MT转录，用镉-血红蛋白法测定MT蛋白。采集贵州省中国砷中毒患者和健康人的血样和口腔细胞标本，提取总RNA进行MT转录分析。大鼠和小鼠的血液MT-1和MT-2转录本与相应的肝、肾MT转录本水平呈正相关。此外，这些动物的血液MT-1和MT-2转录本与组织MT蛋白水平呈正相关。人血MT和口腔细胞MT转录本水平也呈正相关。MT-1A和MT-2A是人类血液和口腔细胞中的主要亚型转录本，砷中毒患者MT水平明显低于健康人。血液MT转录本似乎是组织MT水平的有用生物标记物。贵州省砷中毒患者血液中MT转录水平显著降低，这可能是该人群发生砷中毒的原因之一。
Because metallothionein (MT) is a metal-binding protein that protects against metal intoxication, it could be a biomarker for individual sensitivity to metal toxicity. We assessed the use of bloodborne MT transcript as a reflection of tissue MT levels and examined the potential role of MT in arsenic toxicity in an environmentally exposed human population. Rodents were treated with zinc or nonmetallic MT inducers for 4 days, and the blood and tissues were collected for MT transcript analysis by real-time reverse transcriptase–polymerase chain reaction and MT protein determination by the cadmium–hemoglobin assay. Blood and buccal cell samples were collected from arsenicosis patients and healthy subjects residing in Guizhou, China, and total RNA was isolated for MT transcript analysis. There was a positive correlation between blood MT-1 and MT-2 transcripts and corresponding hepatic or renal MT transcript levels in rats and mice. Furthermore, there was a positive correlation between blood MT-1 and MT-2 transcript and tissue MT protein levels in these animals. A positive correlation also occurred between human blood MT and buccal cell MT transcript levels. MT-1A and MT-2A were the major isoform transcripts in human blood and buccal cells, and significantly lower MT levels were seen in arsenicosis patients compared with healthy subjects. Blood MT transcript appears to be a useful biomarker of tissue MT levels. Arsenicosis patients in Guizhou show significantly lower MT transcript levels in blood, which may have predisposed this population to arsenic intoxication.
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影响因子：
10.4
作者：
通讯作者：
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影响因子：
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作者：
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通讯作者：
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作者：
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通讯作者：
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通讯作者：
Stillman, Martin J.
影响因子：
4.5
作者：
Park, JD;Liu, YP;Klaassen, CD
通讯作者：
Klaassen, CD