The maize INDETERMINATE1 flowering time regulator defines a highly conserved zinc finger protein family in higher plants.
The maize INDETERMINATE1 flowering time regulator defines a highly conserved zinc finger protein family in higher plants.
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玉米不确定的1开花时间调节剂定义了高等植物中高度保守的锌指蛋白家族。
DOI：
10.1186/1471-2164-7-158
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发表时间：
2006-06-19
期刊：
影响因子：
4.4
通讯作者：
Mable BK
中科院分区：
文献类型：
--
作者：
Colasanti J;Tremblay R;Wong AY;Coneva V;Kozaki A;Mable BK
玉米INDETERMINATE1基因ID1是玉米开花转换的关键调控基因，也是转录因子基因家族的创始成员，该家族编码一种具有不同排列的锌指结构的蛋白质。锌指及其周围的序列组成了签名ID结构域(IDD)，它似乎存在于所有高等植物基因组中。锌指结构域的存在和先前的生化研究表明ID1与DNA结合，这表明该基因家族的成员参与了转录调控。将在拟南芥和水稻基因组中发现的IDD基因与玉米EST和基因组数据库中发现的所有IDD基因进行比较，表明ID1是该基因家族中唯一的成员。来自玉米、水稻和拟南芥的所有IDD基因之间高度的序列相似性表明它们来自共同的祖先。ID1的几个独特特征表明，它是玉米IDD家族的一个不同成员。虽然在拟南芥基因组中没有明确的ID1同源基因被鉴定出来，但从水稻和高粱中分离到了高度相似的基因，这些基因编码的蛋白质的同源性超出了ID结构域。系统发育比较表明，这些假定的同源基因与玉米ID1一起，形成了一个独立于其他IDD基因的组。与仅在未成熟叶片中检测到的ID1基因不同，几个玉米IDD基因在不同组织中有广泛的表达。此外，用与ID1蛋白和来自水稻和高粱的潜在同源蛋白交叉反应的抗体进行的Western分析表明，所有这三种蛋白都只在未成熟的叶片中检测到。比较基因组分析表明，IDD锌指家族在单子叶植物和双子叶植物中都高度保守。叶片特异的ID1表达模式使其有别于所研究的其他玉米IDD基因。在水稻和高粱中推测的ID1蛋白同源基因也观察到了相似的叶片特异性定位模式。ID1与其他禾本科植物中密切相关的基因之间的这些相似性表明，它们在功能上可能存在相似之处。
The maize INDETERMINATE1 gene, ID1, is a key regulator of the transition to flowering and the founding member of a transcription factor gene family that encodes a protein with a distinct arrangement of zinc finger motifs. The zinc fingers and surrounding sequence make up the signature ID domain (IDD), which appears to be found in all higher plant genomes. The presence of zinc finger domains and previous biochemical studies showing that ID1 binds to DNA suggests that members of this gene family are involved in transcriptional regulation. Comparison of IDD genes identified in Arabidopsis and rice genomes, and all IDD genes discovered in maize EST and genomic databases, suggest that ID1 is a unique member of this gene family. High levels of sequence similarity amongst all IDD genes from maize, rice and Arabidopsis suggest that they are derived from a common ancestor. Several unique features of ID1 suggest that it is a divergent member of the maize IDD family. Although no clear ID1 ortholog was identified in the Arabidopsis genome, highly similar genes that encode proteins with identity extending beyond the ID domain were isolated from rice and sorghum. Phylogenetic comparisons show that these putative orthologs, along with maize ID1, form a group separate from other IDD genes. In contrast to ID1 mRNA, which is detected exclusively in immature leaves, several maize IDD genes showed a broad range of expression in various tissues. Further, Western analysis with an antibody that cross-reacts with ID1 protein and potential orthologs from rice and sorghum shows that all three proteins are detected in immature leaves only. Comparative genomic analysis shows that the IDD zinc finger family is highly conserved among both monocots and dicots. The leaf-specific ID1 expression pattern distinguishes it from other maize IDD genes examined. A similar leaf-specific localization pattern was observed for the putative ID1 protein orthologs from rice and sorghum. These similarities between ID1 and closely related genes in other grasses point to possible similarities in function.
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影响因子：
5.8
作者：
Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
64.8
作者：
Matsumoto, T;Wu, JZ;Sugiura, M
通讯作者：
Sugiura, M
影响因子：
14.9
作者：
Kozaki, A;Hake, S;Colasanti, J
通讯作者：
Colasanti, J
DOI：
10.1073/pnas.94.13.6809
发表时间：
1997-06-24
影响因子：
11.1
作者：
Gaut, BS;Doebley, JF
通讯作者：
Doebley, JF
影响因子：
64.5
作者：
Colasanti, J;Yuan, Z;Sundaresan, V
通讯作者：
Sundaresan, V