Localization of the Escherichia coli cell division protein FtsI (PBP3) to the division site and cell pole
Localization of the Escherichia coli cell division protein FtsI (PBP3) to the division site and cell pole
复制标题
大肠杆菌细胞分裂蛋白 FtsI (PBP3) 定位于分裂位点和细胞极
作者：
D. Weiss;K. Pogliano;M. Carson;L. Guzman;C. Fraipont;M. Nguyen;R. Losick;J. Beckwith
FtsI，也被称为青霉素结合蛋白3，是大肠杆菌分裂隔膜中合成肽聚糖所需的转肽酶。据估计，每个细胞约有100个FtsI分子，远低于免疫电子显微镜的检测极限。在这里，我们证实了FtsI的低丰度，并使用免疫荧光显微镜（一种高度敏感的技术）来显示FtsI在细胞生长的后期阶段定位于分裂部位。有时在细胞极也观察到FtsI；极地定位没有被预料到，它的意义也不为人所知。我们得出结论：(1)免疫荧光显微镜可用于定位丰度低至每个细胞约100个分子的蛋白质；（ii） FtsI活性在间隔形成过程中的时空调节，至少部分是通过蛋白质定时定位到分裂位点来实现的。
FtsI, also known as penicillin‐binding protein 3, is a transpeptidase required for the synthesis of peptidoglycan in the division septum of the bacterium, Escherichia coli. FtsI has been estimated to be present at about 100 molecules per cell, well below the detection limit of immunoelectron microscopy. Here, we confirm the low abundance of FtsI and use immunofluorescence microscopy, a highly sensitive technique, to show that FtsI is localized to the division site during the later stages of cell growth. FtsI was also sometimes observed at the cell pole; polar localization was not anticipated and its significance is not known. We conclude (i) that immunofluorescence microscopy can be used to localize proteins whose abundance is as low as approximately 100 molecules per cell; and (ii) that spatial and temporal regulation of FtsI activity in septum formation is achieved, at least in part, by timed localization of the protein to the division site.
DOI：
10.1073/pnas.94.2.559
发表时间：
1997-01-21
影响因子：
11.1
作者：
Pogliano, J;Pogliano, K;Beckwith, J
通讯作者：
Beckwith, J
影响因子：
10.5
作者：
Levin, PA;Losick, R
通讯作者：
Losick, R
酵母的免疫荧光方法。
影响因子：
--
作者：
Pringle,JR;Adams,AE;Drubin,DG;Haarer,BK
通讯作者：
Haarer,BK