Taxonomic resolutions based on 18S rRNA genes: a case study of subclass copepoda.
Taxonomic resolutions based on 18S rRNA genes: a case study of subclass copepoda.
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基于 18S rRNA 基因的分类学分辨率：桡足亚纲的案例研究。
DOI：
10.1371/journal.pone.0131498
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Wu S;Xiong J;Yu Y
Biodiversity studies are commonly conducted using 18S rRNA genes. In this study, we compared the inter-species divergence of variable regions (V1–9) within the copepod 18S rRNA gene, and tested their taxonomic resolutions at different taxonomic levels. Our results indicate that the 18S rRNA gene is a good molecular marker for the study of copepod biodiversity, and our conclusions are as follows: 1) 18S rRNA genes are highly conserved intra-species (intra-species similarities are close to 100%); and could aid in species-level analyses, but with some limitations; 2) nearly-whole-length sequences and some partial regions (around V2, V4, and V9) of the 18S rRNA gene can be used to discriminate between samples at both the family and order levels (with a success rate of about 80%); 3) compared with other regions, V9 has a higher resolution at the genus level (with an identification success rate of about 80%); and 4) V7 is most divergent in length, and would be a good candidate marker for the phylogenetic study of Acartia species. This study also evaluated the correlation between similarity thresholds and the accuracy of using nuclear 18S rRNA genes for the classification of organisms in the subclass Copepoda. We suggest that sample identification accuracy should be considered when a molecular sequence divergence threshold is used for taxonomic identification, and that the lowest similarity threshold should be determined based on a pre-designated level of acceptable accuracy.
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影响因子：
16.6
作者：
Fonseca, Vera G.;Carvalho, Gary R.;Sung, Way;Johnson, Harriet F.;Power, Deborah M.;Neill, Simon P.;Packer, Margaret;Blaxter, Mark L.;Lambshead, P. John D.;Thomas, W. Kelley;Creer, Simon
通讯作者：
Creer, Simon
影响因子：
4.9
作者：
Gazis, Romina;Rehner, Stephen;Chaverri, Priscila
通讯作者：
Chaverri, Priscila
影响因子：
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作者：
Gattolliat, Jean-Luc;Monaghan, Michael T.
通讯作者：
Monaghan, Michael T.
影响因子：
4.1
作者：
Blanco-Bercial, Leocadio;Bradford-Grieve, Janet;Bucklin, Ann
通讯作者：
Bucklin, Ann
其他证据为中国南方海域巴非亚属 Protapes（软体动物、双壳纲、金龟科）的物种多样性提供了新的见解
影响因子：
1.6
作者：
Chen, Jun;Li, Qi;Yu, Xue
通讯作者：
Yu, Xue