Second-generation environmental sequencing unmasks marine metazoan biodiversity.
Second-generation environmental sequencing unmasks marine metazoan biodiversity.
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DOI：
10.1038/ncomms1095
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发表时间：
2010-10-19
影响因子：
16.6
通讯作者：
Creer, Simon
中科院分区：
文献类型：
--
作者：
Fonseca, Vera G.;Carvalho, Gary R.;Sung, Way;Johnson, Harriet F.;Power, Deborah M.;Neill, Simon P.;Packer, Margaret;Blaxter, Mark L.;Lambshead, P. John D.;Thomas, W. Kelley;Creer, Simon
生物多样性对生态系统的功能、可持续性和复原力至关重要，但海洋多样性在各种空间和分类尺度上的规模和组织尚不明确。在本文中，我们使用第二代测序揭示了苏格兰温带底栖生态系统中假定的多种海洋后生动物生物多样性。我们的研究表明，在微地理尺度上，物种多样性存在显著差异，并反驳了目前公认的小动物群特征、等级丰度和营养动力学相关的生态和分类学范式。当前基于并行ultrasequence分析的分类单元操作聚类的丰富度估计与来源于形态评估的估计大致一致。然而，许多门的分类单元稀疏曲线的斜率仍然不完整，这表明真正的α多样性可能超过目前的认知。这些方法提供了一个快速、客观和具有成本效益的分类框架，用于探索所有迄今为止难以处理但具有生态重要性的群落的生态系统结构和功能之间的联系。测序技术的最新发展为研究生态系统的生物多样性提供了机会。这种宏基因组方法，结合分类群聚类，在这里被用来证明苏格兰海洋群落的物种丰富度比预期的要大得多。
Biodiversity is of crucial importance for ecosystem functioning, sustainability and resilience, but the magnitude and organization of marine diversity at a range of spatial and taxonomic scales are undefined. In this paper, we use second-generation sequencing to unmask putatively diverse marine metazoan biodiversity in a Scottish temperate benthic ecosystem. We show that remarkable differences in diversity occurred at microgeographical scales and refute currently accepted ecological and taxonomic paradigms of meiofaunal identity, rank abundance and concomitant understanding of trophic dynamics. Richness estimates from the current benchmarked Operational Clustering of Taxonomic Units from Parallel UltraSequencing analyses are broadly aligned with those derived from morphological assessments. However, the slope of taxon rarefaction curves for many phyla remains incomplete, suggesting that the true alpha diversity is likely to exceed current perceptions. The approaches provide a rapid, objective and cost-effective taxonomic framework for exploring links between ecosystem structure and function of all hitherto intractable, but ecologically important, communities. Recent developments in sequencing technologies have provided the opportunity to investigate the biodiversity of ecosystems. Such a metagenomic approach, combined with taxon clustering, is used here to demonstrate that the species richness of a marine community in Scotland is much greater than anticipated.
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影响因子：
12.3
作者：
Huse SM;Huber JA;Morrison HG;Sogin ML;Welch DM
通讯作者：
Welch DM
影响因子：
56.9
作者：
Huber, Julie A.;Mark Welch, David;Sogin, Mitchell L.
通讯作者：
Sogin, Mitchell L.
影响因子：
5.1
作者：
Huse SM;Welch DM;Morrison HG;Sogin ML
通讯作者：
Sogin ML
影响因子：
2.5
作者：
Bhadury, Punyasloke;Austen, Melanie C.;Smerdon, Gary R.
通讯作者：
Smerdon, Gary R.
影响因子：
64.8
作者：
Desnues, Christelle;Rodriguez-Brito, Beltran;Rohwer, Forest
通讯作者：
Rohwer, Forest