Evaluation of Auditory Brainstem Response in Chicken Hatchlings.
Evaluation of Auditory Brainstem Response in Chicken Hatchlings.
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DOI：
10.3791/63477
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发表时间：
2022-04-01
影响因子：
1.2
通讯作者：
Sanchez, Jason Tait
中科院分区：
文献类型：
--
作者：
Ordiway, George;McDonnell, Miranda;Mohan, Sandesh;Sanchez, Jason Tait
听觉脑干反应（ABR）在临床听力学、非人类动物和人类研究中是一种非常宝贵的检测方法。尽管在其他脊椎动物模型系统中广泛使用abr来测量听觉神经同步性和估计听力敏感性，但在鸡中记录abr的方法近40年来尚未报道。鸡提供了一个强大的动物研究模型，因为它们的听觉系统在胚胎后期和早期孵化阶段接近功能成熟。我们已经演示了使用皮下针电极阵列在雏鸡中引发单通道或双通道ABR记录的方法。无论电极记录配置（即蒙太奇）如何，ABR记录在超阈值点击刺激的前6毫秒内包括3-4个正向峰值波形。在高强度水平下，峰谷波形幅度为2-11 μV，正峰表现出预期的延迟-强度函数（即延迟随强度降低而增加）。标准化的耳机位置对于最佳录音至关重要，因为松散的皮肤会阻塞耳道，动物运动可能会使刺激传感器脱落。随着动物体温的降低，峰值振幅变小，潜伏期延长，支持维持生理体温的需要。雏鸟（孵化后第1天<3 h）的阈值比成年雏鸟提高了~5 dB，峰值潜伏期增加了~1 ~ 2 ms，峰谷振幅降低了~1 μV。这提示潜在的导电性相关问题（即中耳腔积液），应在幼雏中考虑。总的来说，这里概述的ABR方法可以准确和可重复地记录小鸡的体内听觉功能，可以应用于不同的发育阶段。这些发现很容易与人类和哺乳动物的听力损失、衰老或其他与听觉相关的操纵模型进行比较。我们采用标准的听觉脑干反应（ABR）技术，并将其应用于雏鸡，这是一种听觉功能的早熟鸟类模型。该方案详细概述了动物准备和ABR获取技术，其步骤可转化为其他鸟类或啮齿动物模型。
The auditory brainstem response (ABR) is an invaluable assay in clinical audiology, non-human animals, and human research. Despite the widespread use of ABRs in measuring auditory neural synchrony and estimating hearing sensitivity in other vertebrate model systems, methods for recording ABRs in the chicken have not been reported in nearly four decades. Chickens provide a robust animal research model because their auditory system is near functional maturation during late embryonic and early hatchling stages. We have demonstrated methods used to elicit one or two-channel ABR recordings using subdermal needle electrode arrays in chicken hatchlings. Regardless of electrode recording configuration (i.e., montage), ABR recordings included 3–4 positive-going peak waveforms within the first 6 ms of a suprathreshold click stimulus. Peak-to-trough waveform amplitudes ranged from 2–11 μV at high-intensity levels, with positive peaks exhibiting expected latency-intensity functions (i.e., increase in latency as a function of decreased intensity). Standardized earphone position was critical for optimal recordings as loose skin can occlude the ear canal, and animal movement can dislodge the stimulus transducer. Peak amplitudes were smaller, and latencies were longer as animal body temperature lowered, supporting the need for maintaining physiological body temperature. For young hatchlings (<3 h post-hatch day 1), thresholds were elevated by ~5 dB, peak latencies increased ~1–2 ms, and peak to trough amplitudes were decreased ~1 μV compared to older hatchlings. This suggests a potential conductive-related issue (i.e., fluid in the middle ear cavity) and should be considered for young hatchlings. Overall, the ABR methods outlined here permit accurate and reproducible recording of in-vivo auditory function in chicken hatchlings that could be applied to different stages of development. Such findings are easily compared to human and mammalian models of hearing loss, aging, or other auditory-related manipulations. We have used standard auditory brainstem response (ABR) techniques and applied them to hatchling chickens, a precocious avian model for auditory function. The protocol outlines animal preparation and ABR acquisition techniques in detail, with steps that could translate to other avian or rodent models.
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影响因子：
5.3
作者：
Hong H;Rollman L;Feinstein B;Sanchez JT
通讯作者：
Sanchez JT
影响因子：
2.4
作者：
Brittan-Powell, EF;Lohr, B;Dooling, RJ
通讯作者：
Dooling, RJ
DOI：
10.1080/19336950.2017.1348870
发表时间：
2017-09-03
期刊：
Channels (Austin, Tex.)
影响因子：
--
作者：
Kopp-Scheinpflug C
通讯作者：
Kopp-Scheinpflug C
DOI：
10.1016/0300-9629(92)90248-o
发表时间：
1992-09-01
期刊：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY
影响因子：
--
作者：
KAISER, A
通讯作者：
KAISER, A
影响因子：
4.4
作者：
Bruzual, JJ;Peak, SD;Peebles, ED
通讯作者：
Peebles, ED