Cytomegalovirus-Specific Immunity Recovers More Slowly after Cord Blood Transplantation Compared with Matched Sibling Donor Allogeneic Transplantation.
Cytomegalovirus-Specific Immunity Recovers More Slowly after Cord Blood Transplantation Compared with Matched Sibling Donor Allogeneic Transplantation.
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脐带血移植后巨细胞病毒特异性免疫功能恢复比同胞供体同种异体移植慢
DOI：
10.1016/j.jtct.2020.11.014
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发表时间：
2021-03
影响因子：
3.2
通讯作者：
Brunstein CG
中科院分区：
文献类型：
--
作者：
Bejanyan N;Vlasova-St Louis I;Mohei H;Cao Q;El Jurdi N;Wagner JE;Miller JS;Brunstein CG
据报道，脐带血（UCB）与匹配的兄弟姐妹供体（MSD）造血细胞移植（HCT）相比，NK细胞的定量恢复迅速，但t细胞亚群的恢复较慢，并伴有频繁的病毒感染。然而，与MSD HCT相比，病毒感染倾向的增加是否也是UCB后病毒特异性免疫恢复较慢的结果尚不清楚。我们使用HCT后不同时间点收集的患者血液样本，检测UCB （N=17）与MSD （N=9）后病毒特异性外周血单个核细胞（PBMC）功能的差异。采用干扰素-γ (IFN-γ)酶联免疫吸收点（ELISpot）法测定5种常见病毒的11种免疫肽对分泌IFN-γ的PBMC频率的影响。我们纳入了接受相同的低强度调节方案的患者，没有ATG，没有全身性糖皮质激素，HCT后1年内没有复发或急性/慢性移植物抗宿主病。HCT后CMV血清阳性患者的CMV反应性PBMC频率高于血清阴性患者。在CMV血清阳性的患者中，在一年的HCT中，UCB后CMV反应性PBMC的频率低于MSD。我们观察到在UCB和MSD之间，病毒特异性PBMC对HHV6、EBV、BK和腺病毒抗原的反应没有差异。我们的数据表明，与具有类似恢复的其他病毒相比，在UCB和MSD HCT的CMV血清阳性受体中，CMV特异性免疫的重建速度较慢。这些研究结果支持实施更有效的预防策略，以防止接受UCB HCT的巨细胞病毒血清阳性患者的巨细胞病毒再激活。
Rapid quantitative recovery of NK cells but slower recovery of T-cell subsets along with frequent viral infections are reported after umbilical cord blood (UCB) compared with matched sibling donor (MSD) hematopoietic cell transplantation (HCT). However, it remains unclear whether increased propensity for viral infections is also a result of slower recovery of virus-specific immunity after UCB as compared to MSD HCT. We examined the differences in the function of virus-specific peripheral blood mononuclear cells (PBMC) after UCB (N=17) vs. MSD (N=9) using previously collected patient blood samples at various time points after HCT. Interferon-gamma (IFN-γ) enzyme-linked immune absorbent spot (ELISpot) assay was used to quantify the PBMC frequencies that secrete IFN-γ in response to 11 immunopeptides from 5 common viruses. We included the patients who received the same reduced intensity conditioning regimen without ATG, no systemic glucocorticoids and had no relapse or acute/chronic graft-versus-host disease within 1 year after HCT. The CMV-reactive PBMC frequencies were higher in CMV seropositive vs. seronegative patients after HCT. Among CMV seropositive patients, the frequency of CMV-reactive PBMC was lower after UCB compared to MSD throughout one year of HCT. We observed no differences in virus-specific PBMC responses towards HHV6, EBV, BK, and adenovirus antigens between UCB and MSD. Our data demonstrate that the reconstitution of CMV-specific immunity is slower in CMV seropositive recipients of UCB vs. MSD HCT in contrast to other viruses which had similar recoveries. These study findings support implementation of more potent prophylactic strategies for preventing CMV reactivation in CMV seropositive patients receiving UCB HCT.
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DOI：
10.1016/j.bbmt.2015.02.017
发表时间：
2015-07
期刊：
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation
影响因子：
--
作者：
Saliba RM;Rezvani K;Leen A;Jorgensen J;Shah N;Hosing C;Parmar S;Oran B;Olson A;Rondon G;Chen J;Martinez C;Hamdi A;Mehta RS;Chemaly RF;Saunders IM;Bollard CM;Shpall EJ
通讯作者：
Shpall EJ
影响因子：
39.2
作者：
Aldoss, Ibrahim;La Rosa, Corinna;Baden, Lindsey R.;Longmate, Jeffrey;Ariza-Heredia, Ella J.;Rida, Wasima N.;Lingaraju, Chetan Raj;Zhou, Qiao;Martinez, Joy;Kaltcheva, Teodora;Dagis, Andy;Hardwick, Nicola;Issa, Nicolas C.;Farol, Len;Nademanee, Auayporn;Al Malki, Monzr M.;Forman, Stephen;Nakamura, Ryotaro;Diamond, Don J.
通讯作者：
Diamond, Don J.
影响因子：
20.3
作者：
Leen, Ann M.;Bollard, Catherine M.;Heslop, Helen E.
通讯作者：
Heslop, Helen E.
影响因子：
4.3
作者：
Johnsrud, Joyce J.;Nguyen, Isabelle T.;Brown, Janice (Wes)
通讯作者：
Brown, Janice (Wes)
影响因子：
4.3
作者：
Jacobson, Caron A.;Turki, Amin T.;Ritz, Jerome
通讯作者：
Ritz, Jerome