The joint evolutionary histories of Wolbachia and mitochondria in Hypolimnas bolina.
The joint evolutionary histories of Wolbachia and mitochondria in Hypolimnas bolina.
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DOI：
10.1186/1471-2148-9-64
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发表时间：
2009-03-24
影响因子：
3.4
通讯作者：
Hurst GD
中科院分区：
文献类型：
--
作者：
Charlat S;Duplouy A;Hornett EA;Dyson EA;Davies N;Roderick GK;Wedell N;Hurst GD
蓝月亮蝴蝶，Hypolimnas bolina，和沃尔巴克氏体之间的相互作用引起了人们的兴趣，因为高患病率的男性杀死内实现的物种，这种高患病率的生态后果，选择的强度对主机，以抑制感染，并存在多种沃尔巴克氏体感染诱导不同的表型。我们研究了共同遗传标记，mtDNA的多样性，以及不同感染状态的个体之间的分区，作为一种手段来调查沃尔巴克氏体感染的种群生物学和进化历史。部分线粒体COI基因测序从298个已知感染状态的个体揭示10种不同的单倍型。尽管有非常强有力的生物学证据表明该样本代表一个单一物种，但这10个单倍型并不属于Hypolimnas属内的单系分支，其中一个单倍型与其他9个单倍型相差5%。感染状况与mtDNA单倍型之间有很强的相关性。wBol 1感染与强烈分歧的单倍型的存在，促使wBol 1遗传变异的更密切的检查。这揭示了两种隐藏亚型wBol 1a和wBol 1b的存在。到目前为止，最常见的wBol 1a感染与单一的不同mtDNA单倍型密切相关。wBol 1b感染被发现有两个单倍型，也在未感染的标本中观察到。最后，wBol 2感染与大量的mtDNA单倍型的多样性，最经常与未感染的同域蝴蝶共享。这一数据总体上支持了以下假设，即在H. bolina与非常高的传播效率而不是常规的水平传播有关。这也表明这种感染经历了最近的选择性扫描，并通过渐渗引入该物种。相比之下，wBol 2感染和未感染个体之间的单倍型共享表明该毒株不是完全传播的和/或显示出显著水平的水平传播。
The interaction between the Blue Moon butterfly, Hypolimnas bolina, and Wolbachia has attracted interest because of the high prevalence of male-killing achieved within the species, the ecological consequences of this high prevalence, the intensity of selection on the host to suppress the infection, and the presence of multiple Wolbachia infections inducing different phenotypes. We examined diversity in the co-inherited marker, mtDNA, and the partitioning of this between individuals of different infection status, as a means to investigate the population biology and evolutionary history of the Wolbachia infections. Part of the mitochondrial COI gene was sequenced from 298 individuals of known infection status revealing ten different haplotypes. Despite very strong biological evidence that the sample represents a single species, the ten haplotypes did not fall within a monophyletic clade within the Hypolimnas genus, with one haplotype differing by 5% from the other nine. There were strong associations between infection status and mtDNA haplotype. The presence of wBol1 infection in association with strongly divergent haplotypes prompted closer examination of wBol1 genetic variation. This revealed the existence of two cryptic subtypes, wBol1a and wBol1b. The wBol1a infection, by far the most common, was in strict association with the single divergent mtDNA haplotype. The wBol1b infection was found with two haplotypes that were also observed in uninfected specimens. Finally, the wBol2 infection was associated with a large diversity of mtDNA haplotypes, most often shared with uninfected sympatric butterflies. This data overall supports the hypothesis that high prevalence of male-killing Wolbachia (wBol1) in H. bolina is associated with very high transmission efficiency rather than regular horizontal transmission. It also suggests this infection has undergone a recent selective sweep and was introduced in this species through introgression. In contrast, the sharing of haplotypes between wBol2-infected and uninfected individuals indicates that this strain is not perfectly transmitted and/or shows a significant level of horizontal transmission.
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影响因子：
5.8
作者：
Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
9.8
作者：
Hornett EA;Charlat S;Duplouy AM;Davies N;Roderick GK;Wedell N;Hurst GD
通讯作者：
Hurst GD
影响因子：
3.8
作者：
Dyson, EA;Kamath, MK;Hurst, GDD
通讯作者：
Hurst, GDD
DOI：
10.1111/j.1095-8312.1996.tb01446.x
发表时间：
1996-08-01
影响因子：
1.9
作者：
Johnstone, RA;Hurst, GDD
通讯作者：
Hurst, GDD
影响因子：
9.2
作者：
Charlat, Sylvain;Reuter, Max;Hurst, Gregory D. D.
通讯作者：
Hurst, Gregory D. D.