Electron acceptors for anaerobic oxidation of methane drive microbial community structure and diversity in mud volcanoes
Electron acceptors for anaerobic oxidation of methane drive microbial community structure and diversity in mud volcanoes
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甲烷厌氧氧化的电子受体驱动泥火山微生物群落结构和多样性
DOI：
10.1111/1462-2920.14128
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发表时间：
2018-07
影响因子：
5.1
通讯作者：
Fortin Danielle
中科院分区：
文献类型：
--
作者：
Ren Ge;Ma Anzhou;Zhang Yanfen;Deng Ye;Zheng Guodong;Zhuang Xuliang;Zhuang Guoqiang;Fortin Danielle
泥火山(MVS)在全球范围内向大气中排放了大量甲烷，然而，由于微生物及其相关的生物地球化学过程的作用在很大程度上被忽视，人们对这种环境中的甲烷循环还没有完全了解。在这里，我们使用来自中国西北部准噶尔盆地6个MVS的微生物16S rRNA基因扩增的高通量测序数据来量化多样性格局，并表征古菌和细菌的群落结构。我们在所有样品中都发现了厌氧甲烷氧化菌和不同的硫酸盐和铁还原微生物，古生菌和细菌群落的多样性与硫酸盐、铁和硝酸盐的浓度密切相关，它们可以作为甲烷厌氧氧化(AOM)的电子受体。通过微观世界实验验证了硫酸盐/铁/硝酸盐对MVS中AOM的影响。在此基础上，选取了两个具有代表性的微生物种群，从系统发育分子生态网络的角度探讨了微生物之间的相互作用。这些地点显示出不同的网络结构、关键物种和微生物相互作用，在富含铁/硫酸盐的MV中观察到甲烷循环微生物与其伙伴之间更复杂和更多的联系。这些发现表明，电子受体是推动这些甲烷丰富环境中微生物群落结构的重要因素。
Mud volcanoes (MVs) emit globally significant quantities of methane into the atmosphere, however, methane cycling in such environments is not yet fully understood, as the roles of microbes and their associated biogeochemical processes have been largely overlooked. Here, we used data from high-throughput sequencing of microbial 16S rRNA gene amplicons from six MVs in the Junggar Basin in northwest China to quantify patterns of diversity and characterize the community structure of archaea and bacteria. We found anaerobic methanotrophs and diverse sulfate- and iron-reducing microbes in all of the samples, and the diversity of both archaeal and bacterial communities was strongly linked to the concentrations of sulfate, iron and nitrate, which could act as electron acceptors in anaerobic oxidation of methane (AOM). The impacts of sulfate/iron/nitrate on AOM in the MVs were verified by microcosm experiments. Further, two representative MVs were selected to explore the microbial interactions based on phylogenetic molecular ecological networks. The sites showed distinct network structures, key species and microbial interactions, with more complex and numerous linkages between methane-cycling microbes and their partners being observed in the iron/sulfate-rich MV. These findings suggest that electron acceptors are important factors driving the structure of microbial communities in these methane-rich environments.
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影响因子：
6.4
作者：
Zhou, Jizhong;Deng, Ye;Zhi, Xiaoyang
通讯作者：
Zhi, Xiaoyang
DOI：
10.1038/ismej.2014.217
发表时间：
2015-06
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.2
作者：
Sharp CE;Stott MB;Dunfield PF
通讯作者：
Dunfield PF
影响因子：
--
作者：
Shejiao Wang;Shengbiao Hu;Tiejun Li;Jiyang Wang;Wenzhi Zhao
通讯作者：
Shejiao Wang;Shengbiao Hu;Tiejun Li;Jiyang Wang;Wenzhi Zhao
影响因子：
56.9
作者：
Beal, Emily J.;House, Christopher H.;Orphan, Victoria J.
通讯作者：
Orphan, Victoria J.