Spatial scales of bacterial community diversity at cold seeps (Eastern Mediterranean Sea).
Spatial scales of bacterial community diversity at cold seeps (Eastern Mediterranean Sea).
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DOI：
10.1038/ismej.2014.217
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发表时间：
2015-06
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影响因子：
--
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中科院分区：
文献类型：
--
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冷渗漏是在海底碳氢化合物排放路径周围形成的高产、破碎的海洋生态系统。微生物利用甲烷和其他碳氢化合物的产物为这些地点丰富的化学合成群落提供燃料，与周围的深海海底相比，它们的呼吸速率要高得多。然而，与非渗水群落相比，冷渗水细菌群落的丰富度、组成和空间尺度尚不清楚。在这里，我们评估了东地中海深海和周围海底地区9个不同冷渗漏的细菌多样性。群落相似性分析基于自动核糖体基因间间隔分析（ARISA）指纹图谱和高通量454标签测序，并结合原位和非原位地球化学分析，跨越几十米到数百公里的空间尺度。渗漏群落以Deltaproteobacteria、Epsilonproteobacteria和Gammaproteobacteria为主，与附近非渗漏深海沉积物群落共有36%的细菌类型（ARISA OTUs）。渗水沉积物的细菌群落与非渗水沉积物有显著差异。在几十到几百米的空间尺度上，冷渗区细菌群落差异很大，在ARISA OTU水平上共享小于50%的类型。它们的变化反映了烃类厌氧降解产生的孔隙水中硫化物浓度的差异。该研究表明，冷渗生态系统对深海微生物多样性有重要贡献。
Cold seeps are highly productive, fragmented marine ecosystems that form at the seafloor around hydrocarbon emission pathways. The products of microbial utilization of methane and other hydrocarbons fuel rich chemosynthetic communities at these sites, with much higher respiration rates compared with the surrounding deep-sea floor. Yet little is known as to the richness, composition and spatial scaling of bacterial communities of cold seeps compared with non-seep communities. Here we assessed the bacterial diversity across nine different cold seeps in the Eastern Mediterranean deep-sea and surrounding seafloor areas. Community similarity analyses were carried out based on automated ribosomal intergenic spacer analysis (ARISA) fingerprinting and high-throughput 454 tag sequencing and were combined with in situ and ex situ geochemical analyses across spatial scales of a few tens of meters to hundreds of kilometers. Seep communities were dominated by Deltaproteobacteria, Epsilonproteobacteria and Gammaproteobacteria and shared, on average, 36% of bacterial types (ARISA OTUs (operational taxonomic units)) with communities from nearby non-seep deep-sea sediments. Bacterial communities of seeps were significantly different from those of non-seep sediments. Within cold seep regions on spatial scales of only tens to hundreds of meters, the bacterial communities differed considerably, sharing <50% of types at the ARISA OTU level. Their variations reflected differences in porewater sulfide concentrations from anaerobic degradation of hydrocarbons. This study shows that cold seep ecosystems contribute substantially to the microbial diversity of the deep-sea.
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DOI：
10.1073/pnas.0803070105
发表时间：
2008-06-03
影响因子：
11.1
作者：
Fuhrman, Jed A.;Steele, Joshua A.;Brown, James H.
通讯作者：
Brown, James H.
影响因子：
4.5
作者：
Glud, Ronnie N.;Stahl, Henrik;Kitazato, Hiroshi
通讯作者：
Kitazato, Hiroshi
影响因子：
4.5
作者：
Felden, J.;Wenzhoefer, F.;Boetius, A.
通讯作者：
Boetius, A.
影响因子：
3.7
作者：
Bienhold C;Pop Ristova P;Wenzhöfer F;Dittmar T;Boetius A
通讯作者：
Boetius A
影响因子：
8.8
作者：
Anderson, Marti J.;Crist, Thomas O.;Swenson, Nathan G.
通讯作者：
Swenson, Nathan G.