Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS.
Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS.
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DOI：
10.1007/s00216-015-8585-6
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发表时间：
2015-07
影响因子：
4.3
通讯作者：
Armirotti, Andrea
中科院分区：
文献类型：
--
作者：
Basit, Abdul;Piomelli, Daniele;Armirotti, Andrea
我们报告了一种新的，灵敏的，快速的LC-MS/MS方法，用于同时测定人血浆中的25种关键鞘脂组分，包括磷酸化鞘氨醇和鞘氨醇，在一个9分钟的运行。该方法能够有效和高通量地覆盖生理或病理状态期间涉及鞘脂组的代谢变化。该方法是基于液-液萃取，然后进行反相LC-MS/MS。外源奇数链脂质被用作具有成本效益但可靠的内标物。根据FDA指南，在替代基质和未处理人血浆中对该方法进行了充分验证。还检测并验证了样品稳定性和稀释完整性。本文的在线版本（doi：10.1007/s 00216 -015-8585-6）包含补充材料，可供授权用户使用。
We report on a new, sensitive, and fast LC-MS/MS method for the simultaneous determination of 25 key sphingolipid components in human plasma, including phosphorylated sphinganine and sphingosine, in a single 9-min run. This method enables an effective and high-throughput coverage of the metabolic changes involving the sphingolipidome during physiological or pathological states. The method is based on liquid–liquid extraction followed by reversed-phase LC-MS/MS. Exogenous odd-chain lipids are used as cost-effective but reliable internal standards. The method was fully validated in surrogate matrix and naive human plasma following FDA guidelines. Sample stability and dilution integrity were also tested and verified. The online version of this article (doi:10.1007/s00216-015-8585-6) contains supplementary material, which is available to authorized users.
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DOI：
10.1016/j.bbalip.2011.05.011
发表时间：
2011-11-01
影响因子：
4.8
作者：
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通讯作者：
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影响因子：
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通讯作者：
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影响因子：
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