Reversible contraceptive effect of PH-20 immunization in male guinea pigs.
Reversible contraceptive effect of PH-20 immunization in male guinea pigs.
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PH-20 免疫对雄性豚鼠的可逆避孕作用。
DOI：
10.1095/biolreprod56.5.1142
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发表时间：
1997
影响因子：
3.6
通讯作者：
Myles,DG
中科院分区：
文献类型：
--
作者：
Primakoff,P;Woolman-Gamer,L;Tung,KS;Myles,DG
目前正在研究精子蛋白作为一种避孕疫苗的抗原。精子质膜蛋白提供了免疫男性或女性和达到避孕效果的理论上的可能性。在本研究中，我们研究了精子质膜蛋白PH-20作为诱导雄性不育的抗原。我们发现，用纯化PH-20免疫的雄性豚鼠可以再现性地诱导不育：100%（29 / 29）接种PH-20的雄性豚鼠不育，而所有22只对照均可生育。雄鼠对PH-20免疫反应极好：单次注射5µg PH-20即可诱导不育。在体重为300克时接受首次注射的男性中，15人中有14人在首次注射后约1年恢复了生育能力。令人惊讶的是，在另一组体重为650克（体重）时接受第一次注射的雄性中，只有1 / 5的雄性在首次注射后约1年恢复了生育能力。两次注射的男性抗血清（首次注射后2个月）抗ph -20滴度一般在1.1-4.2 × 104之间，一次注射的男性抗血清滴度在1.8-9.4 × 103之间。在接下来的6-11个月里，两次注射的雄性滴度下降了4倍以上，而一次注射的雄性滴度略有下降（1.1- 1.8倍）。6-11个月后，抗ph -20滴度在1.0-4.8 × 103范围内，精确残留滴度与生育/不育无关。结果表明，雄性接种PH-20，即使是低剂量，也能产生可重复的、完全有效的避孕作用。
Sperm proteins are currently being studied as antigens on which to base a contraceptive vaccine. Sperm plasma membrane proteins offer the theoretical possibility of immunizing either males or females and achieving a contraceptive effect. In this study, we investigated the sperm plasma membrane protein PH-20 as an antigen for inducing infertility in males. We found that infertility can reproducibly be induced in male guinea pigs immunized with purified PH-20: 100% (29 of 29) of PH-20-immunized males became infertile, whereas all 22 controls were fertile. The males were extremely responsive to PH-20 immunization: infertility could be induced with a single injection of only 5 µg PH-20. Among males that received their initial injection when they were ∼300 g (body weight), 14 of 15 had regained fertility at about 1 yr after initial injection. Surprisingly, in another group of males that received their first injection when they were ∼650 g (body weight), only 1 of 5 had regained fertility about 1 yr after initial injection. Anti-PH-20 titers in antisera (2 mo after initial injection) were generally in the range 1.1–4.2 x 104in twice-injected males and the range 1.8–9.4 x 103in once-injected males. Over the next 6–11 mo, twice-injected males' titers decreased ≥ 4-fold, whereas once-injected males' titers decreased slightly (1.1- to 1.8-fold). After 6–11 mo, anti-PH-20 titers were in the range 1.0–4.8 x 103, and the precise residual titer did not correlate with fertility/infertility. The results show that immunization of males with PH-20, even at low doses, results in a reproducible, completely effective contraceptive action.
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