Bicelles: A natural 'molecular goniometer' for structural, dynamical and topological studies of molecules in membranes.
Bicelles: A natural 'molecular goniometer' for structural, dynamical and topological studies of molecules in membranes.
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DOI：
10.1016/j.biochi.2009.02.003
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发表时间：
2009-06
期刊：
影响因子：
3.9
通讯作者：
Dufourc, Erick J.
中科院分区：
文献类型：
--
作者：
Diller, Anna;Loudet, Cecile;Aussenac, Fabien;Raffard, Gerard;Fournier, Sylvie;Laguerre, Michel;Grelard, Axelle;Opella, Stanley J.;Marassi, Francesca M.;Dufourc, Erick J.
关键词：
主要的生物过程发生在生物膜上。最大的挑战之一是了解膜内化学或生物分子的功能，以及那些参与膜运输的分子。这需要获得一个完整的图片，在原位结构和动力学，以及这些分子在膜脂质双层的拓扑结构和方向。这些导致了生物膜的几种创新模型的创建，以研究两亲分子的结构和动力学，以及具有单个或多个跨膜片段的整合膜蛋白。由于膜中分子的结构、动力学和拓扑结构的确定需要系统的宏观排列，一种新的膜模型称为“双胞”，它代表了脂质囊泡和经典胶束之间的交叉，由于其在磁场中自发自取向的性质而变得非常流行。此外，在模拟天然膜中涉及的关键因素，如样品水合、pH和盐度限制，在双胞系统中易于控制。双胞由长链（14-18个碳）和短链磷脂（6-8个碳）的混合物组成，用缓冲液水合高达98%，并可根据脂质组成、温度和水合作用采用各种形态。我们一直在开发由具有饱和或含联苯的脂肪酰基链的脂质制成的纳米盘形式的bicelle系统。根据脂质的性质，这些膜状纳米盘可以宏观取向，其法线垂直或平行于磁场，为结构和拓扑研究提供天然的“分子测角器”，特别是在NMR领域。双胞也可以以魔角旋转，并导致膜中分子的3D结构确定。
Major biological processes occur at the biological membrane. One of the great challenges is to understand the function of chemical or biological molecules inside the membrane; as well of those involved in membrane trafficking. This requires obtaining a complete picture of the in situ structure and dynamics as well as the topology and orientation of these molecules in the membrane lipid bilayer. These led to the creation of several innovative models of biological membranes in order to investigate the structure and dynamics of amphiphilic molecules, as well as integral membrane proteins having single or multiple transmembrane segments. Because the determination of the structure, dynamics and topology of molecules in membranes requires a macroscopic alignment of the system, a new membrane model called ‘bicelles’ that represents a crossover between lipid vesicles and classical micelles has become very popular due to its property of spontaneous self-orientation in magnetic fields. In addition, crucial factors involved in mimicking natural membranes, such as sample hydration, pH and salinity limits, are easy to control in bicelle systems. Bicelles are composed of mixtures of long chain (14–18 carbons) and short chain phospholipids (6–8 carbons) hydrated up to 98% with buffers and may adopt various morphologies depending on lipid composition, temperature and hydration. We have been developing bicelle systems under the form of nano-discs made of lipids with saturated or biphenyl-containing fatty acyl chains. Depending on the lipid nature, these membranous nano-discs may be macroscopically oriented with their normal perpendicular or parallel to the magnetic field, providing a natural ‘molecular goniometer’ for structural and topological studies, especially in the field of NMR. Bicelles can also be spun at the magic angle and lead to the 3D structural determination of molecules in membranes.
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影响因子：
2.7
作者：
Losonczi, JA;Prestegard, JH
通讯作者：
Prestegard, JH
DOI：
10.1073/pnas.051629298
发表时间：
2001-02-27
影响因子：
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作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
3.9
作者：
Aussenac, F;Laguerre, M;Dufourc, EJ
通讯作者：
Dufourc, EJ