Genetic analysis of an F(2) intercross between two chicken lines divergently selected for body-weight.
Genetic analysis of an F(2) intercross between two chicken lines divergently selected for body-weight.
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DOI：
10.1186/1471-2164-10-248
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发表时间：
2009-05-27
期刊：
影响因子：
4.4
通讯作者：
Andersson L
中科院分区：
文献类型：
--
作者：
Wahlberg P;Carlborg O;Foglio M;Tordoir X;Syvänen AC;Lathrop M;Gut IG;Siegel PB;Andersson L
我们进行了数量性状基因座（QTL）分析的F2杂交两个鸡系分歧选择少年体重。在以前的研究中，13个基因座对体重有影响，只能解释F2群体中大变异的一小部分。上位性互作分析表明，亲本品系间的体重差异是由一个效应较大的互作位点网络造成的。然而，先前的分析是基于稀疏的微卫星连锁图，覆盖范围有限可能影响了主要结论。在这里，我们提出了一个修订的QTL分析的基础上，提供了一个更完整的覆盖范围的鸡基因组的高密度连锁图。此外，我们利用来自约13，000个SNP的基因型数据来搜索基因组，以寻找所选品系中发生的潜在选择性扫描。我们构建了一个包含434个遗传标记的连锁图谱，覆盖了31条染色体，但留下7条微染色体未被覆盖。分析表明，7个地区的QTL，影响生长。成对相互作用分析确定了15个独特的QTL对，值得注意的是，其中9个涉及与7号染色体上的位点的相互作用，形成相互作用位点的网络。对~ 13，000个SNP的分析表明，在两个选择的品系中的任一个中，创始群体中存在的遗传变异的相当大的比例已经丢失，因为品系之间的~60%的SNP多态性在其中一个品系中显示出固定。利用目前的标记覆盖率和QTL图谱分辨率，我们没有观察到QTL间隔内选择性扫描的明显迹象。利用改进的连锁图进行QTL分析的结果与我们以前对该家系的分析结果在很大程度上一致。亲本间的体重差异是由多个QTL引起的，每个QTL的个体效应很小。虽然增加的染色体标记覆盖率并没有导致额外的QTL的鉴定，我们能够细化QTL的定位。上位性相互作用的重要性，作为一种机制，显着贡献显着的选择反应得到进一步加强，因为额外的相互作用位点对检测与改进的地图。
We have performed Quantitative Trait Loci (QTL) analysis of an F2 intercross between two chicken lines divergently selected for juvenile body-weight. In a previous study 13 identified loci with effects on body-weight, only explained a small proportion of the large variation in the F2 population. Epistatic interaction analysis however, indicated that a network of interacting loci with large effect contributed to the difference in body-weight of the parental lines. This previous analysis was, however, based on a sparse microsatellite linkage map and the limited coverage could have affected the main conclusions. Here we present a revised QTL analysis based on a high-density linkage map that provided a more complete coverage of the chicken genome. Furthermore, we utilized genotype data from ~13,000 SNPs to search the genome for potential selective sweeps that have occurred in the selected lines. We constructed a linkage map comprising 434 genetic markers, covering 31 chromosomes but leaving seven microchromosomes uncovered. The analysis showed that seven regions harbor QTL that influence growth. The pair-wise interaction analysis identified 15 unique QTL pairs and notable is that nine of those involved interactions with a locus on chromosome 7, forming a network of interacting loci. The analysis of ~13,000 SNPs showed that a substantial proportion of the genetic variation present in the founder population has been lost in either of the two selected lines since ~60% of the SNPs polymorphic among lines showed fixation in one of the lines. With the current marker coverage and QTL map resolution we did not observe clear signs of selective sweeps within QTL intervals. The results from the QTL analysis using the new improved linkage map are to a large extent in concordance with our previous analysis of this pedigree. The difference in body-weight between the parental chicken lines is caused by many QTL each with a small individual effect. Although the increased chromosomal marker coverage did not lead to the identification of additional QTL, we were able to refine the localization of QTL. The importance of epistatic interaction as a mechanism contributing significantly to the remarkable selection response was further strengthened because additional pairs of interacting loci were detected with the improved map.
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