Routine saliva testing for SARS-CoV-2 in children: Methods for partnering with community childcare centers.
Routine saliva testing for SARS-CoV-2 in children: Methods for partnering with community childcare centers.
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DOI：
10.3389/fpubh.2023.1003158
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发表时间：
2023
影响因子：
5.2
通讯作者：
Bei, Amy K.
中科院分区：
文献类型：
--
作者：
Rayack, Erica J.;Askari, Hibah Mahwish;Zirinsky, Elissa;Lapidus, Sarah;Sheikha, Hassan;Peno, Chikondi;Kazemi, Yasaman;Yolda-Carr, Devyn;Liu, Chen;Grubaugh, Nathan D.;Ko, Albert I.;Wyllie, Anne L.;Spatz, Erica S.;Oliveira, Carlos R.;Bei, Amy K.
虽然相当多的注意力放在SARS-CoV-2检测和监测方案在K-12设置，在儿童保育中心的年轻年龄组在很大程度上被忽视。儿童保育设施对社区至关重要，使父母/监护人能够继续工作，并为儿童和工作人员提供安全的环境。因此，在2019冠状病毒病大流行初期（2020年10月），我们在托儿设施建立了基于PCR的2019冠状病毒病监测计划，对儿童和员工进行检测，旨在收集可操作的公共卫生数据，并帮助社区逐步恢复标准运营和生活方式。在这项研究中，我们描述了每周唾液检测计划的发展，并提供了我们在托儿中心实施这一计划的经验的早期结果。我们招募了7家儿童保育机构的儿童（6个月至7岁）和工作人员，并对参与者进行了使用视频聊天技术收集唾液的培训。每周进行一次调查，以评估暴露、症状和疫苗接种状态的变化。参与者每周在学校提交唾液样本。样本被运送到合作的临床实验室或使用SalivaDirect进行RT-PCR检测，结果在24小时内上传到每个参与者的在线患者门户网站。SARS-CoV-2筛查和常规检测计划对儿童保育人群的关注较少，导致这一关键年龄组的知识差距，特别是因为许多人仍然没有资格接种疫苗。SARS-CoV-2的唾液直接检测为儿童和托儿中心工作人员提供了一种可行的无症状筛查和症状检测方法。考虑到年轻人群对鼻拭子的相对厌恶，家庭唾液收集方法提供了一种有吸引力的替代方法，特别是作为常规监测工具。结果可以通过参与者的私人医疗图表门户网站以电子方式快速共享，视频聊天技术允许研究人员和参与者之间进行讨论和指导。本研究旨在促进参与的托儿中心、家长/监护人和工作人员之间的合作伙伴关系，以减少COVID-19在托儿中心的传播。通过与父母/监护人合作，构思新的收集策略，并为父母/监护人提供持续的虚拟指导和支持，可以克服唾液收集中与唾液有关的挑战。
While considerable attention was placed on SARS-CoV-2 testing and surveillance programs in the K-12 setting, younger age groups in childcare centers were largely overlooked. Childcare facilities are vital to communities, allowing parents/guardians to remain at work and providing safe environments for both children and staff. Therefore, early in the COVID-19 pandemic (October 2020), we established a PCR-based COVID-19 surveillance program in childcare facilities, testing children and staff with the goal of collecting actionable public health data and aiding communities in the progressive resumption of standard operations and ways of life. In this study we describe the development of a weekly saliva testing program and provide early results from our experience implementing this in childcare centers. We enrolled children (aged 6 months to 7 years) and staff at seven childcare facilities and trained participants in saliva collection using video chat technology. Weekly surveys were sent out to assess exposures, symptoms, and vaccination status changes. Participants submitted weekly saliva samples at school. Samples were transported to a partnering clinical laboratory or RT-PCR testing using SalivaDirect and results were uploaded to each participant's online patient portal within 24 h. SARS-CoV-2 screening and routine testing programs have focused less on the childcare population, resulting in knowledge gaps in this critical age group, especially as many are still ineligible for vaccination. SalivaDirect testing for SARS-CoV-2 provides a feasible method of asymptomatic screening and symptomatic testing for children and childcare center staff. Given the relative aversion to nasal swabs in younger age groups, an at-home saliva collection method provides an attractive alternative, especially as a routine surveillance tool. Results can be shared rapidly electronically through participants' private medical chart portals, and video chat technology allows for discussion and instruction between investigators and participants. This study fosters a cooperative partnership with participating childcare centers, parents/guardians, and staff with the goal of mitigating COVID-19 transmission in childcare centers. Age-related challenges in saliva collection can be overcome by working with parents/guardians to conceptualize new collection strategies and by offering parents/guardians continued virtual guidance and support.
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DOI：
10.1016/j.medj.2020.12.010
发表时间：
2021-03-12
期刊：
Med (New York, N.Y.)
影响因子：
--
作者：
Vogels CBF;Watkins AE;Harden CA;Brackney DE;Shafer J;Wang J;Caraballo C;Kalinich CC;Ott IM;Fauver JR;Kudo E;Lu P;Venkataraman A;Tokuyama M;Moore AJ;Muenker MC;Casanovas-Massana A;Fournier J;Bermejo S;Campbell M;Datta R;Nelson A;Yale IMPACT Research Team;Dela Cruz CS;Ko AI;Iwasaki A;Krumholz HM;Matheus JD;Hui P;Liu C;Farhadian SF;Sikka R;Wyllie AL;Grubaugh ND
通讯作者：
Grubaugh ND
影响因子：
11.8
作者：
Ott IM;Strine MS;Watkins AE;Boot M;Kalinich CC;Harden CA;Vogels CBF;Casanovas-Massana A;Moore AJ;Muenker MC;Nakahata M;Tokuyama M;Nelson A;Fournier J;Bermejo S;Campbell M;Datta R;Dela Cruz CS;Farhadian SF;Ko AI;Iwasaki A;Grubaugh ND;Wilen CB;Wyllie AL;Yale IMPACT Research team3
通讯作者：
Yale IMPACT Research team3
影响因子：
4.5
作者：
Harris PA;Taylor R;Thielke R;Payne J;Gonzalez N;Conde JG
通讯作者：
Conde JG
影响因子：
8.9
作者：
Dhiman N;Miller RM;Finley JL;Sztajnkrycer MD;Nestler DM;Boggust AJ;Jenkins SM;Smith TF;Wilson JW;Cockerill FR 3rd;Pritt BS
通讯作者：
Pritt BS
影响因子：
4.5
作者：
Harris, Paul A.;Taylor, Robert;Duda, Stephany N.
通讯作者：
Duda, Stephany N.