Effectiveness of patient-collected swabs for influenza testing.
Effectiveness of patient-collected swabs for influenza testing.
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DOI：
10.1016/j.mayocp.2012.02.011
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发表时间：
2012-06
影响因子：
8.9
通讯作者：
Pritt BS
中科院分区：
文献类型：
--
作者：
Dhiman N;Miller RM;Finley JL;Sztajnkrycer MD;Nestler DM;Boggust AJ;Jenkins SM;Smith TF;Wilson JW;Cockerill FR 3rd;Pritt BS
比较自我采集和医护人员（HCW）采集鼻拭子检测流感病毒的有效性，并确定患者对采集类型的偏好。我们招募了2011年1月28日至2011年4月30日在明尼苏达州罗切斯特的马约诊所急诊科就诊的流感样疾病成人患者。患者按照书面说明从其右鼻孔自行采集中鼻甲鼻植绒拭子。然后由HCW从左心室采集第二个拭子。通过实时逆转录-聚合酶链反应检测拭子中的甲型和B型流感病毒，并确定采集方法之间的一致性百分比。在分析的72份配对标本中，25份通过至少一种采集方法检测为甲型或B流感病毒RNA阳性（阳性率为34.7%）。当排除14例既往接受过医疗保健培训的患者时，采集方法之间的定性一致率为94.8%（55/58）。58份未经卫生保健培训的患者标本中有2份（3.4%）仅通过HCW采集呈阳性，58份标本中有1份（1.7%）仅通过患者自身采集呈阳性。共有53.4%的患者（31/58）更喜欢自我收集方法，而不是HCW收集，25.9%的患者（15/58）没有偏好。自我采集的中鼻甲鼻拭子为甲型和B型流感病毒实时逆转录-聚合酶链反应提供了HCW采集的可靠替代方法。
To compare the effectiveness of self-collected and health care worker (HCW)–collected nasal swabs for detection of influenza viruses and determine the patients' preference for type of collection. We enrolled adult patients presenting with influenzalike illness to the Emergency Department at Mayo Clinic, Rochester, Minnesota, from January 28, 2011, through April 30, 2011. Patients self-collected a midturbinate nasal flocked swab from their right nostril following written instructions. A second swab was then collected by an HCW from the left nostril. Swabs were tested for influenza A and B viruses by real-time reverse transcription–polymerase chain reaction, and percent concordance between collection methods was determined. Of the 72 paired specimens analyzed, 25 were positive for influenza A or B RNA by at least one of the collection methods (34.7% positivity rate). When the 14 patients who had prior health care training were excluded, the qualitative agreement between collection methods was 94.8% (55 of 58). Two of the 58 specimens (3.4%) from patients without health care training were positive only by HCW collection, and 1 of 58 (1.7%) was positive only by patient self-collection. A total of 53.4% of patients (31 of 58) preferred the self-collection method over the HCW collection, and 25.9% (15 of 58) had no preference. Self-collected midturbinate nasal swabs provide a reliable alternative to HCW collection for influenza A and B virus real-time reverse transcription–polymerase chain reaction.
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影响因子：
120.7
作者：
Thompson, WW;Shay, DK;Fukuda, K
通讯作者：
Fukuda, K
影响因子：
5.5
作者：
Lambert, Stephen B.;Allen, Kelly M.;Nolan, Terence M.
通讯作者：
Nolan, Terence M.
影响因子：
9.4
作者：
Abu-Diab, Afaf;Azzeh, Maysa;Hindiyeh, Musa Y.
通讯作者：
Hindiyeh, Musa Y.
影响因子：
4.8
作者：
Esposito S;Molteni CG;Daleno C;Valzano A;Tagliabue C;Galeone C;Milani G;Fossali E;Marchisio P;Principi N
通讯作者：
Principi N
影响因子：
9.4
作者：
Heikkinen, T;Marttila, J;Ruuskanen, O
通讯作者：
Ruuskanen, O