Odor-Induced, Activity-Dependent Transneuronal Gene InductionIn Vitro: Mediation by NMDA Receptors
Odor-Induced, Activity-Dependent Transneuronal Gene InductionIn Vitro: Mediation by NMDA Receptors
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气味诱导的、活性依赖性的跨神经元基因诱导体外：NMDA 受体的介导
DOI：
10.1523/jneurosci.19-04-01359.1999
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
M. T. Shipley
中科院分区：
文献类型：
--
作者：
A. Puche;M. T. Shipley
Expression of tyrosine hydroxylase (TH) by juxtaglomerular (JG) neurons of the olfactory bulb (OB) requires innervation of the bulb by olfactory receptor neurons (ORNs). ORN lesion selectively downregulates TH in JG neurons. In reversible odor deprivation, TH expression is downregulated as the naris is closed and then upregulated upon naris reopening. The mechanism or mechanisms regulating this dependence are unknown. TH expression could be regulated by trophic factor release and/or synaptic activity from ORN terminals. We investigated TH expression in cocultures of dissociated postnatal rat OB cells and embryonic olfactory neuroepithelium (OE) slice explants. TH-positive neurons in control dissociated OB cell cultures alone comprise only a small fraction of the total population of cells present in the culture. However, when OE slice explants are cocultured with dispersed OB cells, there is a mean 2.4-fold increase in the number of TH-positive neurons. ORNs in vivo use glutamate as a neurotransmitter. Broad spectrum excitatory amino acid antagonists (kyurenic acid) or selective antagonists of the NMDA receptor (APV) both prevent induction of TH expression in OE–OB cocultures. Furthermore, pulse application of NMDA stimulates TH expression in OB neurons in the absence of OE. In vitro, OB TH neurons express NMDA receptors, suggesting that NMDA stimulation is acting directly on TH neurons. Exposure of OE explants to natural odorants results in upregulation of TH, presumably through increased ORN activity, which could be blocked by APV. These findings indicate that odorant-stimulated glutamate release by ORN terminals regulates TH expression via NMDA receptors on JG dopaminergic neurons.
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DOI：
10.1016/0165-3806(90)90132-i
发表时间：
1990-04
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
S. Pixley;R. Pun
通讯作者：
S. Pixley;R. Pun
影响因子：
2.5
作者：
TROMBLEY, PQ;WESTBROOK, GL
通讯作者：
WESTBROOK, GL
影响因子：
56.9
作者：
BADING, H;GINTY, DD;GREENBERG, ME
通讯作者：
GREENBERG, ME
培养的嗅球神经元细胞内 Ca2 储存的分布和激活。
DOI：
10.1152/jn.1997.78.4.2176
发表时间：
1997
期刊：
Journal of neurophysiology.
影响因子：
--
作者：
Carlson,GC;Slawecki,ML;Lancaster,E;Keller,A
通讯作者：
Keller,A
酪氨酸羟化酶基因的基础转录和诱导转录均依赖于 cAMP 反应元件。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kim,KS;Lee,MK;Carroll,J;Joh,TH
通讯作者：
Joh,TH