Identification, Pathogenicity, and Genetic Diversity of Fusarium spp. Associated with Maize Sheath Rot in Heilongjiang Province, China.
Identification, Pathogenicity, and Genetic Diversity of Fusarium spp. Associated with Maize Sheath Rot in Heilongjiang Province, China.
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镰刀菌属的鉴定、致病性和遗传多样性。与中国黑龙江省玉米鞘腐病有关。
DOI：
10.3390/ijms231810821
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发表时间：
2022-09-16
影响因子：
5.6
通讯作者：
Zhao, Junwei
中科院分区：
文献类型：
--
作者：
Yang, Xilang;Xu, Xi;Wang, Shuo;Zhang, Li;Shen, Guijin;Teng, Haolin;Yang, Chunbo;Song, Chunru;Xiang, Wensheng;Wang, Xiangjing;Zhao, Junwei
玉米鞘腐病是我国玉米的一种流行病害。 2020年至2021年，在黑龙江省玉米主产区采集有症状样本。为了阐明种群和遗传多样性以及导致玉米鞘腐病的病原体的毒力，总共获得了 132 个镰刀菌分离株并用于后续研究。根据形态特征鉴定出10个镰孢菌种，并利用TEF-1α基因序列进行系统发育分析，包括Fusarium verticillioides（50.00%）、F. subglutinans（18.94%）、Fusarium incarnatum-equiseti物种复合体（14.39%）、F. temperatum（5.30%）、F. acuminatum（3.03%）、F. f. F. solani (2.27%)、F. sporotrichioides (2.27%)、F. tricinctum (1.52%)、F. asiaticum (1.52%) 和 F. proliferatum (0.76%)。 10个镰刀菌属均可在玉米鞘上产生椭圆形至环状病斑，病斑中心呈灰黄色至深棕色，周围有深灰色至深棕色晕圈。其中，F. tricinctum 和 F. proliferatum 的毒力明显高于其他镰刀菌属物种。此外，基于ITS和TEF-1a基因串联序列的单倍型分析表明，属于Fusarium fujikuroi物种复合体的99个Fusarium分离物（由F. verticillioides分离物、F. subglutinans分离物、F. temperatum分离物和F. proliferatum分离物组成）可分为10个单倍型，其中包括5个共享单倍型单倍型（Haps 1、2、4、5 和 6）和 5 个私有单倍型（Haps 3、7、8、9 和 10）。此外，单倍型网络中的 F. verticillioides 进化枝以 Hap 2 为中心呈放射状，表明发生了种群扩张。本研究表明，与黑龙江省玉米鞘腐病相关的镰刀菌属种类比以往报道的更加丰富，首次确认F. subglutinans、F. temperatum、F. solani、F. sporotrichioides、F. tricinctum和F. acuminatum为黑龙江省玉米鞘腐病的致病菌。
Maize sheath rot is a prevalent maize disease in China. From 2020 to 2021, symptomatic samples were collected from the main maize-growing regions of Heilongjiang province. To clarify the population and genetic diversity, as well as the virulence of pathogens responsible for maize sheath rot, a total of 132 Fusarium isolates were obtained and used for follow-up studies. Ten Fusarium species were identified based on morphological characteristics, and phylogenetic analysis was conducted using the TEF-1α gene sequences, including F. verticillioides (50.00%), F. subglutinans (18.94%), the Fusarium incarnatum-equiseti species complex (14.39%), F. temperatum (5.30%), F. acuminatum (3.03%), F. solani (2.27%), F. sporotrichioides (2.27%), F. tricinctum (1.52%), F. asiaticum (1.52%), and F. proliferatum (0.76%). All 10 Fusarium species could produce oval-to-annular lesions on maize sheath, and the lesions were grayish yellow to dark brown in the center and surrounded by a dark gray-to-dark brown halo. Of these, F. tricinctum and F. proliferatum showed significantly higher virulence than the other Fusarium species. In addition, haplotype analysis based on the concatenated sequences of the ITS and TEF-1a genes showed that 99 Fusarium isolates which belonged to the Fusarium fujikuroi species complex—consisting of F. verticillioides isolates, F. subglutinans isolates, F. temperatum isolates, and F. proliferatum isolates—could be grouped into 10 haplotypes, including 5 shared haplotypes (Haps 1, 2, 4, 5, and 6) and 5 private haplotypes (Haps 3, 7, 8, 9, and 10). Furthermore, the F. verticillioides clade in the haplotype network was radial with the center of Hap 2, suggesting that population expansion occurred. This research showed that Fusarium species associated with maize sheath rot in Heilongjiang province are more diverse than previously reported, and this is the first time that F. subglutinans, F. temperatum, F. solani, F. sporotrichioides, F. tricinctum, and F. acuminatum have been confirmed as the causal agents of maize sheath rot in Heilongjiang province.
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影响因子：
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作者：
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