Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes.
Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes.
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DOI:
10.1039/c2cc38882k
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发表时间:
2013-02-21
期刊:
影响因子:
--
通讯作者:
Trau M
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文献类型:
--
作者:
Grewal YS;Shiddiky MJ;Gray SA;Weigel KM;Cangelosi GA;Trau M
Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.
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DOI:
10.1073/pnas.0504917102
发表时间:
2005-10-11
影响因子:
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Sensors (Basel, Switzerland)
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