Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes.

Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes.
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DOI:
10.1039/c2cc38882k
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发表时间:
2013-02-21
期刊:
Chemical communications (Cambridge, England)
影响因子:
--
通讯作者:
Trau M
Trau M
中科院分区:
其他
文献类型:
--
作者:
Grewal YS;Shiddiky MJ;Gray SA;Weigel KM;Cangelosi GA;Trau M

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廉价、简单和快速地检测人类样本中的病原体抗原是一个关键的全球卫生目标。限制因素包括利用抗体探针的诊断测试的成本和复杂性。在此,我们提出了一种无标记的电化学检测的蛋白质从肠道病原体溶组织内阿米巴使用无细胞的酵母包埋的抗体样片段(酵母-scFv)作为新的亲和试剂的方法。
Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.
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