Expression analysis of the ligands for the Natural Killer cell receptors NKp30 and NKp44.
Expression analysis of the ligands for the Natural Killer cell receptors NKp30 and NKp44.
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DOI：
10.1371/journal.pone.0001339
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发表时间：
2007-12-19
期刊：
影响因子：
3.7
通讯作者：
Watzl C
中科院分区：
文献类型：
--
作者：
Byrd A;Hoffmann SC;Jarahian M;Momburg F;Watzl C
天然细胞毒性受体（NCR）对于刺激自然杀伤（NK）细胞对抗转化细胞的活性是重要的。NCR配体及其在正常和肿瘤细胞上的表达水平的鉴定对于癌症免疫治疗策略的合理设计具有重要意义。在这里，我们分析了NKp 30配体和NKp 44配体在30个不同来源的转化或非转化细胞系上的表达。我们发现这两种配体在几乎所有测试的细胞系上的细胞内和表面表达。NKp 30和NKp 44配体的表达是可变的，与细胞系的来源无关。NKp 30和NKp 44配体的表达分别与NKp 30和NKp 44介导的NK细胞溶解肿瘤细胞相关。NKp 30配体和NKp 44配体的表面表达对胰蛋白酶处理敏感，并且在G2/M期停滞的细胞中减少。这些数据证明了NKp 30和NKp 44配体的普遍表达，并对这些配体的调节提供了重要的见解。
The natural cytotoxicity receptors (NCR) are important to stimulate the activity of Natural Killer (NK) cells against transformed cells. Identification of NCR ligands and their level of expression on normal and neoplastic cells has important implications for the rational design of immunotherapy strategies for cancer. Here we analyze the expression of NKp30 ligand and NKp44 ligand on 30 transformed or non-transformed cell lines of different origin. We find intracellular and surface expression of these two ligands on almost all cell lines tested. Expression of NKp30 and NKp44 ligands was variable and did not correlate with the origin of the cell line. Expression of NKp30 and NKp44 ligand correlated with NKp30 and NKp44-mediated NK cell lysis of tumor cells, respectively. The surface expression of NKp30 ligand and NKp44 ligand was sensitive to trypsin treatment and was reduced in cells arrested in G2/M phase. These data demonstrate the ubiquitous expression of the ligands for NKp30 and NKp44 and give an important insight into the regulation of these ligands.
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DOI：
10.1084/jem.188.5.953
发表时间：
1998-09-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Pessino A;Sivori S;Bottino C;Malaspina A;Morelli L;Moretta L;Biassoni R;Moretta A
通讯作者：
Moretta A
影响因子：
5.4
作者：
Chisholm, SE;Reyburn, HT
通讯作者：
Reyburn, HT
影响因子：
20.3
作者：
Nowbakht, P;Ionescu, MCS;Wodnar-Filipowicz, A
通讯作者：
Wodnar-Filipowicz, A
影响因子：
6.4
作者：
Chisholm, Susan E.;Howard, Keith;Reyburn, Hugh T.
通讯作者：
Reyburn, Hugh T.
影响因子：
20.3
作者：
Bryceson, YT;March, ME;Long, EO
通讯作者：
Long, EO