Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough.
Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough.
复制标题
冲绳海槽冷泉沉积物中厌氧甲烷氧化剂的多样性
DOI：
10.3389/fmicb.2022.819187
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发表时间：
2022
影响因子：
5.2
通讯作者：
Zhou, Yucheng
中科院分区：
文献类型：
--
作者：
Chen, Ye;Xu, Cuiling;Wu, Nengyou;Sun, Zhilei;Liu, Changling;Zhen, Yu;Xin, Youzhi;Zhang, Xilin;Geng, Wei;Cao, Hong;Zhai, Bin;Li, Jing;Qin, Shuangshuang;Zhou, Yucheng
冲绳海槽(OT)中活跃的冷渗漏已经被广泛识别，但与这些地点相关的沉积物微生物群落仍然知之甚少。在这里，我们调查了冲绳海槽中部活跃的冷泉沉积物中微生物群落的分布和生物量，特别是与甲烷厌氧氧化(AOM)相关的微生物群落。在OT冷渗漏沉积物中发现了几种甲烷氧化古菌，包括ANME-1a、ANME-1b、ANME-2a/b、ANME-2c和ANME-3。厌氧甲烷营养古菌(ANME)群落的垂直分层顺序为：ANME-3，ANME-1a，ANME-1b。此外，甲基辅酶M还原酶A(MCRA)基因的丰度对应着较高的溶解铁水平，这表明代谢甲烷的古生菌可能参与了OT冷泉中铁还原-甲烷氧化(Fe-AOM)。此外，ANME-1a的相对丰度与溶解铁的浓度密切相关，表明ANME-1a是OT冷渗漏沉积物中Fe-AOM的关键微生物。共生分析表明，甲烷代谢微生物群落主要与异养微生物有关，如JS1、Bathy-1和Bathy-15。
Active cold seeps in the Okinawa Trough (OT) have been widely identified, but the sediment microbial communities associated with these sites are still poorly understood. Here, we investigated the distribution and biomass of the microbial communities, particularly those associated with the anaerobic oxidation of methane (AOM), in sediments from an active cold seep in the mid-Okinawa Trough. Methane-oxidizing archaea, including ANME-1a, ANME-1b, ANME-2a/b, ANME-2c, and ANME-3, were detected in the OT cold seep sediments. Vertical stratification of anaerobic methanotrophic archaea (ANME) communities was observed in the following order: ANME-3, ANME-1a, and ANME-1b. In addition, the abundance of methyl coenzyme M reductase A (mcrA) genes corresponded to high levels of dissolved iron, suggesting that methane-metabolizing archaea might participate in iron reduction coupled to methane oxidation (Fe-AOM) in the OT cold seep. Furthermore, the relative abundance of ANME-1a was strongly related to the concentration of dissolved iron, indicating that ANME-1a is a key microbial player for Fe-AOM in the OT cold seep sediments. Co-occurrence analysis revealed that methane-metabolizing microbial communities were mainly associated with heterotrophic microorganisms, such as JS1, Bathy-1, and Bathy-15.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
56.9
作者：
Beal, Emily J.;House, Christopher H.;Orphan, Victoria J.
通讯作者：
Orphan, Victoria J.
影响因子：
11.4
作者：
Egger, Matthias;Rasigraf, Olivia;Slomp, Caroline P.
通讯作者：
Slomp, Caroline P.
影响因子：
4.5
作者：
Felden, J.;Wenzhoefer, F.;Boetius, A.
通讯作者：
Boetius, A.
影响因子：
4.5
作者：
IVERSEN, N;JORGENSEN, BB
通讯作者：
JORGENSEN, BB