Molecular recognition of methyl α-D-mannopyranoside by antifreeze (glyco)proteins.
Molecular recognition of methyl α-D-mannopyranoside by antifreeze (glyco)proteins.
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DOI：
10.1021/ja502837t
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发表时间：
2014-06-25
影响因子：
15
通讯作者：
Rheingold, Arnold L.
中科院分区：
文献类型：
--
作者：
Wang, Sen;Wen, Xin;DeVries, Arthur L.;Bagdagulyan, Yelena;Morita, Alexander;Golen, James A.;Duman, John G.;Rheingold, Arnold L.
抗冻蛋白和糖蛋白[AF（G）Ps]由于其通过与特定冰晶面结合而抑制冰的生长和重结晶的能力而被人们所熟知，并且它们显示出与特定冰晶面的显著结构相容性。在这里，我们表明，甲基α-d-吡喃甘露糖苷（MDM），一种代表性的吡喃糖，晶体生长面也显示出值得注意的结构相容性与已知的AF（G）Ps的周期性。我们选择鱼AFGPs（AFGP 8，AFGP 1 -5），和甲虫AFP（DAFP 1）具有增加抗冻活性作为潜在的添加剂控制MDM晶体生长。与其对冰生长的影响相似，AF（G）Ps可以抑制MDM晶体的生长和重结晶，更重要的是，AF（G）Ps的效力与其抗冻活性密切相关。在AF（G）Ps存在下生长的MDM晶体更小，具有更好的限定形状，并且如通过单晶X射线衍射和偏光显微镜所示比对照晶体具有更高的质量，但是通过单晶X射线衍射、固态NMR和衰减全反射红外光谱没有鉴定出MDM的新的多晶型物。观察到的MDM晶体的平均尺寸的变化可以与在AF（G）Ps存在下MDM核的数目的变化相关。计算了添加剂存在和不存在时MDM成核的临界自由能变化差。这些值与AF（G）Ps存在下的冰成核值接近，表明AF（G）Ps对MDM的分子识别涉及类似的相互作用。据我们所知，这是第一份报告，其中AF（G）Ps已被用于控制碳水化合物的晶体生长和AFGP控制非冰状晶体。我们的研究结果表明，MDM可能是一个可能的替代冰研究AF（G）P晶体相互作用的详细机制。本文还讨论了AF（G）Ps与糖结合蛋白的关系。AF（G）Ps和生长晶面之间的结构相容性增加了AF（G）Ps的分子识别能力，这可能在糖、食品、制药和材料工业中具有潜在的应用。
Antifreeze proteins and glycoproteins [AF(G)Ps] have been well-known for more than three decades for their ability to inhibit the growth and recrystallization of ice through binding to specific ice crystal faces, and they show remarkable structural compatibility with specific ice crystal faces. Here, we show that the crystal growth faces of methyl α-d-mannopyranoside (MDM), a representative pyranose sugar, also show noteworthy structural compatibility with the known periodicities of AF(G)Ps. We selected fish AFGPs (AFGP8, AFGP1–5), and a beetle AFP (DAFP1) with increasing antifreeze activity as potential additives for controlling MDM crystal growth. Similar to their effects on ice growth, the AF(G)Ps can inhibit MDM crystal growth and recrystallization, and more significantly, the effectiveness for the AF(G)Ps are well correlated with their antifreeze activity. MDM crystals grown in the presence of AF(G)Ps are smaller and have better defined shapes and are of higher quality as indicated by single crystal X-ray diffraction and polarized microscopy than control crystals, but no new polymorphs of MDM were identified by single crystal X-ray diffraction, solid-state NMR, and attenuated total reflectance infrared spectroscopy. The observed changes in the average sizes of the MDM crystals can be related to the changes in the number of the MDM nuclei in the presence of the AF(G)Ps. The critical free energy change differences of the MDM nucleation in the absence and presence of the additives were calculated. These values are close to those of the ice nucleation in the presence of AF(G)Ps suggesting similar interactions are involved in the molecular recognition of MDM by the AF(G)Ps. To our knowledge this is the first report where AF(G)Ps have been used to control crystal growth of carbohydrates and on AFGPs controlling non-ice-like crystals. Our finding suggests MDM might be a possible alternative to ice for studying the detailed mechanism of AF(G)P–crystal interactions. The relationships between AF(G)Ps and carbohydrate binding proteins are also discussed. The structural compatibility between AF(G)Ps and growing crystal faces demonstrated herein adds to the repertoire of molecular recognition by AF(G)Ps, which may have potential applications in the sugar, food, pharmaceutical, and materials industries.
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DOI：
10.1107/s0567740869005267
发表时间：
1969-01-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL CRYSTALLOGRAPHY AND CRYSTAL CHEMISTRY
影响因子：
--
作者：
HUNT, DJ;SUBRAMANIAN, E
通讯作者：
SUBRAMANIAN, E
影响因子：
1.8
作者：
Garnier, S;Petit, S;Coquerel, G
通讯作者：
Coquerel, G
DOI：
10.1073/pnas.1100429108
发表时间：
2011-05-03
影响因子：
11.1
作者：
Garnham, Christopher P.;Campbell, Robert L.;Davies, Peter L.
通讯作者：
Davies, Peter L.
影响因子：
64.8
作者：
KNIGHT, CA;DEVRIES, AL;OOLMAN, LD
通讯作者：
OOLMAN, LD
影响因子：
2.9
作者：
Li, N;Chibber, BAK;Duman, JG
通讯作者：
Duman, JG