The interaction of imposed and inherent olfactory mucosal activity patterns and their composite representation in a mammalian species using voltage-sensitive dyes
The interaction of imposed and inherent olfactory mucosal activity patterns and their composite representation in a mammalian species using voltage-sensitive dyes
复制标题
使用电压敏感染料在哺乳动物物种中强加的和固有的嗅觉粘膜活动模式的相互作用及其复合表示
DOI：
10.1523/jneurosci.16-01-00345.1996
复制
发表时间：
1996
影响因子：
4.8
通讯作者：
DE Hornung
中科院分区：
文献类型：
--
作者：
PF Kent;MM Mozell;SJ Murphy;DE Hornung
从两栖动物的数据中，可以根据气味的粘膜活动模式对气味进行编码的两种机制如下：(1)具有相似气味选择性的受体可以在黏膜上聚集(固有模式)；(2)类似于气相色谱，当气味沿着黏膜表面被吸入时，强吸附的气味可以优先沉积在上游，而弱吸附的气味可以更均匀地分布(强加的模式)。这两种可能的编码机制在哺乳动物中都起作用吗？如果是，它们在给出复合模式(强加的+固有的)时是如何相互作用的？用10×10像素光电二极管阵列监测大鼠粘膜中di-4-ANEPPS的荧光变化。为了观察固有的模式，首先将三种不同吸附能力的气味均匀地喷到安装在Delrin小室中的整个粘膜上。为了带出施加的图案，然后密封房间以解剖复制大鼠的鼻腔，并沿着粘膜流动路径以三种流速吸入这些相同的气味。这一结果首次证明了哺乳动物身上存在强加的模式。与弱吸附的气味不同，强吸附的气味显示出明显的强制模式。在生理范围内，增加流速会降低所施加图案的大小。人们可能会考虑嗅觉过程可以用来否定或利用色层效应的策略，因为复合图案随着刺激条件的变化而不稳定，这引发了关于它们在气味编码中所起作用的问题。
From amphibian data, two mechanisms that could underlie the encoding of odorants by the mucosal activity patterns they engender are as follows (1) receptors with similar odorant selectivities could be aggregated spatially on the mucosa (inherent patterns); (2) in analogy to gas chromatography, as odorants are drawn along the surface of the mucosa the strongly sorbed ones could be deposited preferentially upstream, whereas the weakly sorbed ones could be distributed more evenly (imposed patterns). Do both of these possible coding mechanisms operate in mammals and, if so, how do they interact in giving composite patterns (imposed + inherent)? Fluorescence changes in di-4-ANEPPS applied to rat mucosas were monitored by a 10 x 10 pixel photodiode array. To observe the inherent patterns, three odorants of varying sorbabilities first were puffed uniformly onto the entire mucosa mounted in a Delrin chamber. To bring out the imposed patterns, the chamber was then sealed to replicate anatomically the rat's nasal cavity, and these same odorants were drawn at three flow rates along the mucosal flow path. The results demonstrated for the first time the existence of imposed patterns in a mammal. The strongly sorbed odorants, unlike the weakly sorbed one, showed marked imposed patterns. Within physiological limits, increasing the flow rate decreased the magnitude of the imposed patterns. One might consider strategies that the olfactory process could use either to negate or to take advantage of the chromatographic effect, because the lability of the composite patterns with changing stimulus conditions raises questions about their role in odorant encoding.
大鼠粘膜固有的活动模式：来自电压敏感染料的证据。
影响因子：
2.5
作者：
Youngentob,SL;Kent,PF;Sheehe,PR;Schwob,JE;Tzoumaka,E
通讯作者：
Tzoumaka,E