Human beta-defensin-2 induction in Helicobacter pylori-infected gastric mucosal tissues: antimicrobial effect of overexpression.
Human beta-defensin-2 induction in Helicobacter pylori-infected gastric mucosal tissues: antimicrobial effect of overexpression.
复制标题
幽门螺杆菌感染的胃粘膜组织中人β-防御素-2的诱导：过度表达的抗菌作用。
DOI：
10.1099/jmm.0.04985-0
复制
发表时间：
2003
影响因子：
3
通讯作者：
N. Watanabe
中科院分区：
文献类型：
--
作者：
N. Uehara;A. Yagihashi;K. Kondoh;N. Tsuji;T. Fujita;H. Hamada;N. Watanabe
The objective of this study was to understand more of the innate immune response to Helicobacter pylori by determining the expression of human beta-defensin-2 (hBD-2) in various gastric mucosal tissues and MKN45 gastric cancer cells with or without H. pylori. Semi-quantitative TaqMan RT-PCR and immunohistochemistry were carried out. The antimicrobial effects of a transfected hBD-2 gene against H. pylori were also evaluated. The results showed that hBD-2 was expressed in inflamed gastric mucosal tissues with H. pylori infection, but not in the absence of H. pylori infection. Expression was also detected in gastric cancers in patients with H. pylori infection. Expression was induced in the MKN45 gastric cancer cell line by H. pylori in a manner dependent on the abundance of bacteria. hBD-2-transfected 3T3J2-1 cells secreted hBD-2 protein into the culture medium and this protein inhibited growth of H. pylori completely. The results suggest that hBD-2 may be involved in the pathophysiology of H. pylori-induced gastritis.
影响因子：
2.7
作者：
T. Yajima;A. Yagihashi;H. Kameshima;D. Kobayashi;D. Furuya;K. Hirata;N. Watanabe
通讯作者：
T. Yajima;A. Yagihashi;H. Kameshima;D. Kobayashi;D. Furuya;K. Hirata;N. Watanabe
幽门螺杆菌在 cag 致病岛上的人胃细胞系 MKN45 细胞中诱导人 β-防御素-2 mRNA 表达。
影响因子：
3.1
作者：
Wada,A;Mori,N;Oishi,K;Hojo,H;Nakahara,Y;Hamanaka,Y;Nagashima,M;Sekine,I;Ogushi,K;Niidome,T;Nagatake,T;Moss,J;Hirayama,T
通讯作者：
Hirayama,T