Effects of photodynamic therapy using mono-L-aspartyl chlorin e6 on vessels and its contribution to the antitumor effect.
Effects of photodynamic therapy using mono-L-aspartyl chlorin e6 on vessels and its contribution to the antitumor effect.
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DOI：
10.1111/j.1349-7006.2000.tb00981.x
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发表时间：
2000-05
期刊：
影响因子：
--
通讯作者：
Aizawa K
中科院分区：
文献类型：
--
作者：
Saito K;Mikuniya N;Aizawa K
光动力疗法(PDT)对血管系统的影响在肿瘤组织破坏中具有重要作用。我们研究了血管损伤在PDT抗肿瘤作用中的作用，并分析了PDT后血管的定量变化。给荷纤维肉瘤的BALB/c雄性小鼠注射单剂L天冬氨酸氯乙酯(NPe6)，剂量分别为0.25、5、15 mg/kg，分别于注射后10min、2 h、24 h用半导体激光照射。注射0.25 mg/kg后10min，NPe6仅存在于血浆中，注射5 mg/kg后2 h，NPe6同时存在于血浆和肿瘤中，注射15 mg/kg后24 h，仅存在于肿瘤中。5 mg/kg~2 h组和0.25 mg/kg~10min组的抗肿瘤作用基本相同，15 mg/kg~24 h组的抗肿瘤作用较弱。15 mg/kg~24 h组对肿瘤血管和肿瘤细胞的损伤晚于其他条件下，肿瘤周围组织的血管损伤甚至在PDT后24 h也较轻。这些结果表明，激光照射时的血浆NPe6浓度比肿瘤NPe6浓度对抗肿瘤作用的贡献更大，低浓度的NPe6对肿瘤周围血管的最小损伤可能是未能获得明显的抗肿瘤作用的原因之一。
The effect of photodynamic therapy (PDT) on the vascular system has a significant role in tumor tissue destruction. We investigated the contribution of vascular damage to the antitumor effects of PDT and analyzed the quantitative vascular changes after PDT. Fibrosarcoma‐bearing BALB/c male mice were injected with mono‐L‐aspartyl chlorin e6 (NPe6) at a dose of 0.25, 5 or 15 mg/kg, and photoradiation was performed with a diode laser 10 min, 2 h or 24 h after injection, respectively. Ten minutes after injection of 0.25 mg/kg, NPe6 was found to be present only in plasma, while at 2 h after injection of 5 mg/kg it was present in both plasma and tumor, and 24 h after injection of 15 mg/kg it was present only in the tumor. The antitumor effects observed in the 5 mg/ kg‐2 h and 0.25 mg/kg‐10 min groups were virtually the same, whereas the effect in the 15 mg/kg‐ 24 h group was weaker. The damage to the tumor vasculature and tumor cells in the 15 mg/kg‐24 h group occurred later than under the other conditions, and vascular damage in the tumor‐surrounding tissue was also less marked even 24 h after PDT. These results suggested that the plasma NPe6 concentration during laser irradiation contributed more than the tumor NPe6 concentration to the antitumor effect, and that the minimal damage to blood vessels around the tumor at the low plasma NPe6 concentration may be one reason for the failure to obtain a marked antitumor effect.
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DOI：
10.1111/j.1349-7006.1994.tb02923.x
发表时间：
1994-11-01
期刊：
JAPANESE JOURNAL OF CANCER RESEARCH
影响因子：
--
作者：
KATSUMI, T;AIZAWA, K;KATO, H
通讯作者：
KATO, H
影响因子：
10.3
作者：
ROBERTS, WG;SHIAU, FY;BERNS, MW
通讯作者：
BERNS, MW
影响因子：
3.3
作者：
Lin, GC;Tsoukas, ML;Kollias, N
通讯作者：
Kollias, N
影响因子：
3.3
作者：
ROBERTS, WG;SMITH, KM;BERNS, MW
通讯作者：
BERNS, MW
影响因子：
2.1
作者：
Yamamoto, Y;Shibuya, H;Kato, H
通讯作者：
Kato, H