Human therapeutic cloning, pitfalls and lack luster because of rapid developments in induced pluripotent stem cell technology
Human therapeutic cloning, pitfalls and lack luster because of rapid developments in induced pluripotent stem cell technology
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人类治疗性克隆因诱导多能干细胞技术快速发展而陷入困境且黯淡无光
DOI：
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
K. Sidhu
中科院分区：
文献类型：
--
作者：
S. Hua;Henry C Y Chung;K. Sidhu
摘要背景：治疗性克隆是将体细胞核移植（SCNT）和胚胎干细胞（ES）技术相结合，以创造与患者相匹配的特定ES细胞。由于通过核转移衍生的ES细胞（SCNT ES细胞）与供体在遗传上相同，因此它不会产生宿主免疫系统的排斥反应，因此在治疗上可能更容易接受。诱导性多能干细胞（iPS）是通过诱导一组特定多能基因的强制表达而从成体体细胞人工衍生的一类多能干细胞。在过去的几年里，重编程和iPS技术取得了迅速的进展，这似乎给SCNT计划取得的任何进展蒙上了阴影。目的：本文比较了SCNT和iPS在再生医学中的应用前景。方法：我们使用MEDLINE（PubMed）、Wiley InterScience、Springer、EBSCO和Annual Reviews数据库，使用关键词“iPS”、“ES”、“SCNT”、“诱导多能干细胞”、“胚胎干细胞”、“治疗性克隆”、“再生医学”和“体细胞核移植”进行文献检索。本综述仅纳入英文发表的文章。结果：两种方法各有优缺点。尽管如此，通过使用SCNT生成患者特定的细胞系，它可以避免在iPS生成过程中使用病毒载体，从而消除并发症。老年妇女体外成熟卵子的成功，甚至从生殖干细胞分化卵母细胞，将进一步提高SCNT在再生医学中的应用。结论：在不久的将来，人SCNT可能是产生用于临床治疗的患者干细胞系的适当手段。
Abstract Background: Therapeutic cloning is the combination of somatic cell nuclear transfer (SCNT) and embryonic stem cell (ES) techniques to create specific ES cells that match those of a patient. Because ES cells derived by nuclear transfer (SCNT ES cells) are genetically identical to the donor, it will not generate rejection by the host’s immune system and thus therapeutically may be more acceptable. Induced pluripotent stem cells (iPS) are a type of pluripotent stem cell artificially derived from an adult somatic cell by inducing a forced expression of a set of specific pluripotent genes. In the past few years, rapid progress in reprogramming and iPS technology has been made, and it seems to shadow any progress made in SCNT programs. Objective: This review compares the application perspective of SCNT with that of iPS in regenerative medicine. Methods:We conducted a literature search using the MEDLINE (PubMed), Wiley InterScience, Springer, EBSCO, and Annual Reviews databases using the keywords “iPS”, “ES”, “SCNT” “induced pluripotent stem cells”, “embryonic stem cells”, “therapeutic cloning”, “regenerative medicine”, and “somatic cell nuclear transfer”. Only articles published in English were included in this review. Results: These two methods both have advantages and disadvantages. Nevertheless, by using SCNT to generate patient-specific cell lines, it eliminates complications by avoiding the use of viral vectors during iPS generation. Success in in vitro matured eggs from aged women and even differentiation of oocytes from germ stem cells will further enhance the application of SCNT in regenerative medicine. Conclusion: Human SCNT may be an appropriate mean of generating patient stem cell lines for clinical therapy in the near future.
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影响因子：
4
作者：
Cai, Jingli;Yang, Ming;Iacovitti, Lorraine
通讯作者：
Iacovitti, Lorraine
影响因子：
4
作者：
Wilson, Kitchener D.;Venkatasubrahmanyam, Shivkumar;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
4
作者：
M. Rao;M. Condic
通讯作者：
M. Rao;M. Condic
DOI：
10.1073/pnas.0510485103
发表时间：
2006-01-24
影响因子：
11.1
作者：
Brambrink, T;Hochedlinger, K;Jaenisch, R
通讯作者：
Jaenisch, R
影响因子：
23.9
作者：
Mayshar, Yoav;Ben-David, Uri;Benvenisty, Nissim
通讯作者：
Benvenisty, Nissim