Rare and localized events stabilize microbial community composition and patterns of spatial self-organization in a fluctuating environment.
Rare and localized events stabilize microbial community composition and patterns of spatial self-organization in a fluctuating environment.
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DOI：
10.1038/s41396-022-01189-9
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发表时间：
2022-05
期刊：
影响因子：
11
通讯作者：
Johnson, David R.
中科院分区：
文献类型：
--
作者：
Ciccarese, Davide;Micali, Gabriele;Borer, Benedict;Ruan, Chujin;Or, Dani;Johnson, David R.
空间自组织是表面相关微生物群落的一个标志，它受到当地环境条件的控制，并通过种间相互作用进一步改变。在这里，我们假设微生物细胞类型的空间模式可以在波动的环境条件下稳定交叉喂养微生物群落的组成。我们通过研究由两种代谢相互作用的施氏假单胞菌菌株组成的微生物共培养物的生长和空间自组织来检验这一假设。我们将共培养物接种到琼脂表面，并允许它们扩展（即范围扩展），同时改变环境条件，改变两种菌株之间的依赖性。我们在引起互利相互作用的缺氧条件和引起竞争性相互作用的有氧条件之间交替。我们观察到两种菌株在罕见且高度局部化的集群中同时出现（称为“空间大奖事件”），并且在环境波动期间持续存在。为了解决空间大奖事件出现的潜在机制，我们使用了基于机械主体的数学模型，该模型可以解决与单个细胞相关的规模的生长和扩散。虽然共培养的组成随着互惠相互作用的强度和环境波动而变化，但该模型提供了对空间分辨基质景观形成的见解，这些景观具有支持两种菌株共存和安全共培养功能的局部生态位。这项研究强调，除了响应环境波动而出现的空间模式之外，局部空间大奖事件还确保了压力在动态条件下的持续存在。
Spatial self-organization is a hallmark of surface-associated microbial communities that is governed by local environmental conditions and further modified by interspecific interactions. Here, we hypothesize that spatial patterns of microbial cell-types can stabilize the composition of cross-feeding microbial communities under fluctuating environmental conditions. We tested this hypothesis by studying the growth and spatial self-organization of microbial co-cultures consisting of two metabolically interacting strains of the bacterium Pseudomonas stutzeri. We inoculated the co-cultures onto agar surfaces and allowed them to expand (i.e. range expansion) while fluctuating environmental conditions that alter the dependency between the two strains. We alternated between anoxic conditions that induce a mutualistic interaction and oxic conditions that induce a competitive interaction. We observed co-occurrence of both strains in rare and highly localized clusters (referred to as “spatial jackpot events”) that persist during environmental fluctuations. To resolve the underlying mechanisms for the emergence of spatial jackpot events, we used a mechanistic agent-based mathematical model that resolves growth and dispersal at the scale relevant to individual cells. While co-culture composition varied with the strength of the mutualistic interaction and across environmental fluctuations, the model provides insights into the formation of spatially resolved substrate landscapes with localized niches that support the co-occurrence of the two strains and secure co-culture function. This study highlights that in addition to spatial patterns that emerge in response to environmental fluctuations, localized spatial jackpot events ensure persistence of strains across dynamic conditions.
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影响因子：
7.7
作者：
Gralka, Matti;Hallatschek, Oskar
通讯作者：
Hallatschek, Oskar
影响因子：
11
作者：
Goldschmidt, Felix;Regoes, Roland R.;Johnson, David R.
通讯作者：
Johnson, David R.
DOI：
10.1038/s41396-017-0038-0
发表时间：
2018-03
期刊：
The ISME journal
影响因子：
--
作者：
Chacón JM;Möbius W;Harcombe WR
通讯作者：
Harcombe WR
DOI：
10.1073/pnas.0710150104
发表时间：
2007-12-11
影响因子：
11.1
作者：
Hallatschek, Oskar;Hersen, Pascal;Nelson, David R.
通讯作者：
Nelson, David R.
影响因子：
16.6
作者：
Fusco D;Gralka M;Kayser J;Anderson A;Hallatschek O
通讯作者：
Hallatschek O