The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis.

The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis.
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DOI:
10.1038/s41467-021-23112-0
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发表时间:
2021-05-14
影响因子:
16.6
通讯作者:
De Smet I
De Smet I
中科院分区:
综合性期刊1区
文献类型:
--
作者:
Vu LD;Xu X;Zhu T;Pan L;van Zanten M;de Jong D;Wang Y;Vanremoortele T;Locke AM;van de Cotte B;De Winne N;Stes E;Russinova E;De Jaeger G;Van Damme D;Uauy C;Gevaert K;De Smet I

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植物通过增加器官的伸长生长来增强冷却能力,从而对温和的温暖温度条件做出反应,这一过程称为热形态建成。到目前为止,热形态发生的调节已被专门证明与光信号通路相交。为了确定在开花植物中保守的热形态发生调节因子,我们绘制了暴露于温暖温度的双子叶植物和单子叶植物中蛋白质磷酸化的变化。我们确定丝裂原激活蛋白激酶激酶4(MAP 4K 4)/温度目标3(TOT 3)是热形态建成的调节因子,影响拟南芥中的油菜素类固醇信号传导。此外,我们表明,TOT 3在小麦,单子叶作物的热响应中发挥作用。总而言之,TOT 3保守的热调节扩展了我们对热形态发生的认识,超越了研究充分的途径,有助于确保气候变化下的粮食安全。植物通过称为热形态发生的生长过程对温暖作出反应。在这里,作者通过磷酸蛋白质组学方法表明,有丝分裂原激活的蛋白激酶TOT 3调节小麦和拟南芥的热形态建成,并改变拟南芥中的油菜素类固醇信号传导。
Plants respond to mild warm temperature conditions by increased elongation growth of organs to enhance cooling capacity, in a process called thermomorphogenesis. To this date, the regulation of thermomorphogenesis has been exclusively shown to intersect with light signalling pathways. To identify regulators of thermomorphogenesis that are conserved in flowering plants, we map changes in protein phosphorylation in both dicots and monocots exposed to warm temperature. We identify MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE KINASE4 (MAP4K4)/TARGET OF TEMPERATURE3 (TOT3) as a regulator of thermomorphogenesis that impinges on brassinosteroid signalling in Arabidopsis thaliana. In addition, we show that TOT3 plays a role in thermal response in wheat, a monocot crop. Altogether, the conserved thermal regulation by TOT3 expands our knowledge of thermomorphogenesis beyond the well-studied pathways and can contribute to ensuring food security under a changing climate. Plants respond to warmth via growth processes termed thermomorphogenesis. Here, via a phosphoproteomics approach, the authors show that the mitogen activated protein kinase TOT3 regulates thermomorphogenesis in both wheat and Arabidopsis and modifies brassinosteroid signaling in Arabidopsis.
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