Latest Progress on RNA Methylation Modification in Hepatocellular Carcinoma
Latest Progress on RNA Methylation Modification in Hepatocellular Carcinoma
复制标题
三磷酸腺苷触发的p53基因与阿霉素共传递系统抑制癌细胞增殖
DOI：
10.18063/c
复制
发表时间：
2020-05
期刊：
影响因子：
--
通讯作者：
Tang B
中科院分区：
文献类型：
--
作者：
Dai L;Su H;Shu G;Lai S;Wang Z;Dai H;Tang B
通过刺激响应性纳米载体共递送药物和基因是实现有效癌症治疗的有希望的策略。本研究基于三磷酸腺苷（ATP）触发的核酸适体和聚乙烯亚胺（PEI）构建了一个ATP响应性纳米系统，用于阿霉素（DOX）和p53基因的共递送。在该系统中，DOX与适体及其cDNA序列形成的双链体的富含GC的基序相互作用。然后，以阳离子载体PEI 25 K为载体，构建了DOX-Duplex/PEI/p53三元纳米复合物，以促进p53基因和DOX的细胞内递送和释放。DOX-Duplex/PEI/p53纳米复合物被发现具有有效的抗癌作用，这归因于该系统触发细胞凋亡并同时将细胞周期阻滞在G2期的能力。发现良好的抗增殖作用与响应细胞内ATP浓度的DOX和p53基因快速释放以及治疗药物和基因的协同作用有关。
The codelivery of drugs and genes by stimuli-responsive nanocarriers is a promising strategy for achieving an effective cancer treatment. In this study, an adenosine triphosphate (ATP)-responsive nanosystem was constructed to codeliver doxorubicin (DOX) and p53 gene based on an ATP-triggered aptamer and polyethyleneimine (PEI). In this system, DOX interacts with the GC-rich motif of duplex formed by the aptamer and its cDNA sequence. Then, a ternary nanocomplex DOX-Duplex/PEI/p53 was constructed using cationic carrier PEI25K for facilitating the intracellular delivery and release of p53 gene and DOX. The DOX-Duplex/PEI/p53 nanocomplex was found to possess an efficient anticancer effect, which was attributed to the ability of the system to trigger cell apoptosis and meanwhile block the cell cycle at G2 phase. The favorable antiproliferative effect was found to be associated with the rapid DOX and p53 gene release in response to the intracellular ATP concentration and the synergistic effect of therapeutic drug and gene.
登录
查看更多内容
DOI：
10.1016/j.ymthe.2019.11.021
发表时间：
2019-11
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
Jingshui Xiao;Yankun Liu;Fuxia Wu;Ruiyan Liu;Yongli Xie;Qian Yang;Yu-Feng Li;Min Liu;Sheng-ping Li;Hua Tang
通讯作者：
Jingshui Xiao;Yankun Liu;Fuxia Wu;Ruiyan Liu;Yongli Xie;Qian Yang;Yu-Feng Li;Min Liu;Sheng-ping Li;Hua Tang
影响因子：
1.4
作者：
Yue Zhao;Song You;Ya-qi Yu;S. Zhang;Pengtao Li;Y. Ye;Wen‐xiu Zhao;Jie Li;Qiu‐Shi Li;
通讯作者：
Yue Zhao;Song You;Ya-qi Yu;S. Zhang;Pengtao Li;Y. Ye;Wen‐xiu Zhao;Jie Li;Qiu‐Shi Li;
影响因子：
11.1
作者：
Cheng D;Deng J;Zhang B;He X;Meng Z;Li G;Ye H;Zheng S;Wei L;Deng X;Chen R;Zhou J
通讯作者：
Zhou J
影响因子：
10.5
作者：
Ke S;Alemu EA;Mertens C;Gantman EC;Fak JJ;Mele A;Haripal B;Zucker-Scharff I;Moore MJ;Park CY;Vågbø CB;Kusśnierczyk A;Klungland A;Darnell JE Jr;Darnell RB
通讯作者：
Darnell RB
影响因子：
4.1
作者：
Sloan KE;Warda AS;Sharma S;Entian KD;Lafontaine DLJ;Bohnsack MT
通讯作者：
Bohnsack MT