Quantitative determination of hydroxy fatty acids as an indicator of in vivo lipid peroxidation: oxidation products of arachidonic and docosapentaenoic acids in rat liver after exposure to carbon tetrachloride.
Quantitative determination of hydroxy fatty acids as an indicator of in vivo lipid peroxidation: oxidation products of arachidonic and docosapentaenoic acids in rat liver after exposure to carbon tetrachloride.
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定量测定羟基脂肪酸作为体内脂质过氧化的指标：暴露于四氯化碳后大鼠肝脏中花生四烯酸和二十二碳五烯酸的氧化产物。
DOI：
10.1016/0003-2697(92)90377-j
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发表时间：
1992
影响因子：
2.9
通讯作者：
Stephens,RJ
中科院分区：
文献类型：
--
作者：
Thomas,DW;vanKuijk,FJ;Stephens,RJ
采用改进的气相色谱-质谱联用技术，对四氯化碳刺激的大鼠肝脏脂质过氧化产物进行了定量分析。该方法避免了样品制备过程中不饱和脂肪酸自氧化的问题，并且灵敏度允许对少至1 mg的组织进行测定。这允许从同一器官通过活组织检查获得小的组织样本，从而使得有可能对单个动物进行体内时间研究。从整个组织或细胞制剂中提取脂质，同时通过催化氢化还原，然后皂化并衍生为它们的五氟苄基酯和三甲基甲硅烷基醚。使用氘代化合物或天然存在的脂肪酸代谢物作为内标物，通过负离子化学电离气相色谱-质谱法完成定量。发现由花生四烯酸和二十二碳五烯酸的氢过氧化物的减少产生的羟基脂肪酸在肝脏或肝细胞悬浮液暴露于四氯化碳后20分钟内增加。
An improved gas chromatography-mass spectrometry method has been applied to the quantitation of both in vitro and in vivo products of lipid peroxidation in rat liver stimulated with carbon tetrachloride. The method avoids problems of autoxidation of unsaturated fatty acids during sample preparation, and the sensitivity permits assays on as little as 1 mg of tissue. This permits small samples of tissue to be obtained by biopsy from the same organ, thus making it possible to perform in vivo time studies on a single animal. Lipids from whole tissue or cell preparations are simultaneously extracted and reduced by catalytic hydrogenation and then saponified and derivatized to their pentafluorobenzyl esters and trimethylsilyl ethers. Quantitation is accomplished by negative ion chemical ionization gas chromatography-mass spectrometry, using either deuterated compounds or naturally occurring fatty acid metabolites as internal standards. Hydroxy fatty acids which result from reduction of the hydroperoxides of arachidonic and docosapentaenoic acids are found to increase within 20 min after exposure of liver or hepatocyte suspensions to carbon tetrachloride.
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暴露于不同 CCl4 浓度的离体大鼠肝细胞的超微结构变化。
DOI：
--
发表时间：
1983
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
C. Tyson;D. L. Story;R. J. Stephens
通讯作者：
R. J. Stephens
定量测定羟基脂肪酸作为体内脂质过氧化的指标：气相色谱-质谱法。
影响因子：
2.9
作者：
Thomas,DW;vanKuijk,FJ;Dratz,EA;Stephens,RJ
通讯作者：
Stephens,RJ
DOI：
--
发表时间：
1985
期刊：
Journal of Free Radicals in Biology & Medicine
影响因子：
--
作者：
F. J. V. Kuijk;D. Thomas;D. Thomas;R. Stephens;R. Stephens;Edward A. Dratz
通讯作者：
Edward A. Dratz
花生四烯酸脂氧合酶代谢物五氟苄酯的制备：气相色谱和负离子化学电离质谱分析
DOI：
10.1016/s0378-4347(00)83309-5
发表时间：
1984
期刊：
Journal of Chromatography B: Biomedical Sciences and Applications
影响因子：
--
作者：
R. Strife;R. Murphy
通讯作者：
R. Murphy
高效液相色谱法测定四氯化碳给药后大鼠肝脏磷脂过氧化产物。
影响因子：
3.9
作者：
J. Terao;I. Asano;S. Matsushita
通讯作者：
S. Matsushita