Quantitative determination of hydroxy fatty acids as an indicator of in vivo lipid peroxidation: gas chromatography-mass spectrometry methods.
Quantitative determination of hydroxy fatty acids as an indicator of in vivo lipid peroxidation: gas chromatography-mass spectrometry methods.
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定量测定羟基脂肪酸作为体内脂质过氧化的指标：气相色谱-质谱法。
DOI：
10.1016/0003-2697(91)90513-s
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发表时间：
1991
影响因子：
2.9
通讯作者：
Stephens,RJ
中科院分区：
文献类型：
--
作者：
Thomas,DW;vanKuijk,FJ;Dratz,EA;Stephens,RJ
建立了一种新的脂质过氧化产物的气相色谱-质谱定量分析方法。与现有的气相色谱-质谱法相比，一个重要的优点是消除了样品制备过程中的自氧化。该灵敏度足以允许在正常生理条件下对少至1 mg的组织测量脂质过氧化产物。在提取过程中，通过催化氢化减少来自整个组织样品或细胞制备物的脂质。氢化通过使双键饱和并将氢过氧化物还原成羟基衍生物来稳定化合物。然后将饱和脂质皂化，所得脂肪酸转化为五氟苄基酯。羟基脂肪酸进一步转化为三甲基甲硅烷基醚衍生物。使用氘代内标物，通过负离子化学电离气相色谱-质谱法进行定量。多不饱和脂肪酸的特定产物可以定量，并且该方法区分由自由基和光氧化机制产生的产物。可以测量到由促氧化剂条件（如动物暴露于四氯化碳）导致的脂质过氧化产物水平高于正常生理水平的增加。
A new method has been developed for the quantitation of lipid peroxidation products by gas chromatography-mass spectrometry. An important advantage over existing gas chromatography-mass spectrometry methods is the elimination of autoxidation during sample preparation. The sensitivity is sufficient to permit measurement of lipid peroxidation products under normal physiological conditions on as little as 1 mg of tissue. Lipids from whole tissue samples or cell preparations are reduced by catalytic hydrogenation during extraction. The hydrogenation stabilizes the compounds by saturating the double bonds and reducing the hydroperoxides to hydroxy derivatives. The saturated lipids are then saponified and the resulting fatty acids are converted to pentafluorobenzyl esters. Hydroxy fatty acids are further converted to trimethylsilyl ether derivatives. Quantitation is accomplished by negative ion chemical ionization gas chromatography-mass spectrometry, using deuterated internal standards. Specific products from polyunsaturated fatty acids can be quantitated, and the method differentiates between products produced by free-radical and photooxidation mechanisms. Increased levels of lipid peroxidation products, above normal physiological levels, that result from prooxidant conditions, such as exposure of animals to carbon tetrachloride, can be measured.
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DOI：
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DOI：
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1985
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影响因子：
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DOI：
10.1016/s0378-4347(00)83309-5
发表时间：
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期刊：
Journal of Chromatography B: Biomedical Sciences and Applications
影响因子：
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