Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation.
Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation.
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DOI：
10.1039/c4lc01246a
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发表时间：
2015-03-07
期刊：
影响因子：
6.1
通讯作者：
López GP
中科院分区：
文献类型：
--
作者：
Shields CW 4th;Reyes CD;López GP
准确和高通量的细胞分选是分子和细胞生物学、生物技术和医学中的一项关键使能技术。虽然传统方法可以在短时间内提供高效率的分选，但微流控技术的进步使利用各种物理原理提供类似能力的微型设备得以实现。我们将这些技术分为主动和被动两种。主动系统通常使用外场(例如，声场、电场、磁场和光场)来施加力来移位细胞以进行分选，而被动系统使用惯性力、过滤器和附着机制来净化细胞群。微芯片上的细胞分选通过减小必要设备的尺寸、消除潜在的生物危险气雾剂以及简化通常与细胞分选相关的复杂程序而提供了许多优于传统方法的优点。此外，微芯片器件非常适合并行化，能够实现完整的芯片实验室设备，用于细胞分离、分析和实验处理。在这篇综述中，我们考察了微流控细胞分选技术的广度，同时侧重于那些提供最大潜力转化为临床和工业实践的技术，以及提供多种有用功能的技术。我们根据所需的细胞准备类型(即基于荧光标记的分选、基于珠粒的分选和无标记分选)以及每种分选机制的物理原理来组织这些分选技术。
Accurate and high throughput cell sorting is a critical enabling technology in molecular and cellular biology, biotechnology, and medicine. While conventional methods can provide high efficiency sorting in short timescales, advances in microfluidics have enabled the realization of miniaturized devices offering similar capabilities that exploit a variety of physical principles. We classify these technologies as either active or passive. Active systems generally use external fields (e.g., acoustic, electric, magnetic, and optical) to impose forces to displace cells for sorting, whereas passive systems use inertial forces, filters, and adhesion mechanisms to purify cell populations. Cell sorting on microchips provides numerous advantages over conventional methods by reducing the size of necessary equipment, eliminating potentially biohazardous aerosols, and simplifying the complex protocols commonly associated with cell sorting. Additionally, microchip devices are well suited for parallelization, enabling complete lab-on-a-chip devices for cellular isolation, analysis, and experimental processing. In this review, we examine the breadth of microfluidic cell sorting technologies, while focusing on those that offer the greatest potential for translation into clinical and industrial practice and that offer multiple, useful functions. We organize these sorting technologies by the type of cell preparation required (i.e., fluorescent label-based sorting, bead-based sorting, and label-free sorting) as well as by the physical principles underlying each sorting mechanism.
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影响因子：
8.6
作者：
ASHKIN, A
通讯作者：
ASHKIN, A
影响因子：
6.2
作者：
Chen, Xing;Cui, Dafu;Chen, Jian
通讯作者：
Chen, Jian
影响因子：
1.6
作者：
BONNER, WA;SWEET, RG;HERZENBERG, LA
通讯作者：
HERZENBERG, LA
影响因子：
6.1
作者：
Chen A;Byvank T;Chang WJ;Bharde A;Vieira G;Miller BL;Chalmers JJ;Bashir R;Sooryakumar R
通讯作者：
Sooryakumar R
影响因子：
3.8
作者：
Applegate, RW;Squier, J;Marr, DWM
通讯作者：
Marr, DWM