On-chip magnetic separation and encapsulation of cells in droplets.
On-chip magnetic separation and encapsulation of cells in droplets.
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DOI：
10.1039/c2lc41201b
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发表时间：
2013-03-21
期刊：
影响因子：
6.1
通讯作者：
Sooryakumar R
中科院分区：
文献类型：
--
作者：
Chen A;Byvank T;Chang WJ;Bharde A;Vieira G;Miller BL;Chalmers JJ;Bashir R;Sooryakumar R
由于许多细胞悬浮液的异质性，即使在显著的初始分选之后，对高通量单细胞测定的需求也变得越来越重要。这些悬浮液可能在基因表达水平上显示出细胞与细胞之间的变异性，这可能影响单细胞功能基因组学、癌症、干细胞研究和药物筛选。在隔离环境中对单个细胞的芯片上监测将防止交叉污染，提供高回收率，并能够在单细胞水平上研究生物性状。芯片上生物学实验的这些优点是对传统方法的大量细胞分析的显著改进，传统方法只需要提供关于细胞代谢的平均信息的大量样品。我们报告了一种将移动的磁阱阵列与微流体技术相结合的装置，以提供免疫磁性标记的细胞或磁珠的分离及其与试剂一起封装到皮升液滴中的组合功能。这种在分选后立即进行试剂输送和细胞区室化的方案，全部在同一芯片内无缝进行，提供了独特的优点，例如捕获源自其天然环境的细胞性状的能力，减少污染的机会，仅与分离的物体反应的试剂溶液的最小使用和新鲜度，以及独立于输入流的可调封装特性。除了已证明的初步细胞活力测定外，该设备还可以与其他上游或下游芯片上模块集成，成为强大的单细胞分析工具。
The demand for high-throughput single cell assays is gaining importance because of the heterogeneity of many cell suspensions, even after significant initial sorting. These suspensions may display cell-to-cell variability at the gene expression level that could impact single cell functional genomics, cancer, stem-cell research and drug screening. The on-chip monitoring of individual cells in an isolated environment would prevent cross-contamination, provide high recovery yield, and enable study of biological traits at a single cell level. These advantages of on-chip biological experiments is a significant improvement for myriad of cell analyses over conventional methods, which require bulk samples providing only averaged information on cell metabolism. We report on a device that integrates mobile magnetic trap array with microfluidic technology to provide, combined functionality of separation of immunomagnetically labeled cells or magnetic beads and their encapsulation with reagents into pico-liter droplets. This scheme of simultaneous reagent delivery and compartmentalization of the cells immediately after sorting, all performed seamlessly within the same chip, offers unique advantages such as the ability to capture cell traits as originated from its native environment, reduced chance of contamination, minimal use and freshness of the reagent solution that reacts only with separated objects, and tunable encapsulation characteristics independent of the input flow. In addition to the demonstrated preliminary cell viability assay, the device can potentially be integrated with other up- or downstream on-chip modules to become a powerful single-cell analysis tool.
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影响因子：
46.9
作者：
通讯作者：
--
DOI：
10.1073/pnas.92.3.860
发表时间：
1995-01-31
影响因子：
11.1
作者：
BECKER, FF;WANG, XB;GASCOYNE, PRC
通讯作者：
GASCOYNE, PRC
影响因子：
56.9
作者：
Huang, LR;Cox, EC;Sturm, JC
通讯作者：
Sturm, JC
影响因子：
4
作者：
Adams, Jonathan D.;Thevoz, Patrick;Soh, H. Tom
通讯作者：
Soh, H. Tom
影响因子：
2.8
作者：
Jung, Youngdo;Choi, Yoonsu;Frazier, A. Bruno
通讯作者：
Frazier, A. Bruno