The E3 ligase OsPUB15 interacts with the receptor-like kinase PID2 and regulates plant cell death and innate immunity.
The E3 ligase OsPUB15 interacts with the receptor-like kinase PID2 and regulates plant cell death and innate immunity.
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E3 连接酶 OsPUB15 与受体样激酶 PID2 相互作用，调节植物细胞死亡和先天免疫
DOI：
10.1186/s12870-015-0442-4
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发表时间：
2015-02-13
影响因子：
5.3
通讯作者：
Zhu L
中科院分区：
文献类型：
--
作者：
Wang J;Qu B;Dou S;Li L;Yin D;Pang Z;Zhou Z;Tian M;Liu G;Xie Q;Tang D;Chen X;Zhu L
稻瘟病是世界范围内最具破坏性的水稻病害之一。我们之前克隆了水稻稻瘟病抗性基因epid2，该基因编码一个跨膜受体样激酶，包含细胞外b -凝集素结构域和细胞内丝氨酸/苏氨酸激酶结构域。然而，对pid2介导的信号知之甚少。结果本研究报道了U-box/ARM重复蛋白OsPUB15作为pid2结合蛋白之一的功能表征。我们发现OsPUB15在体外与PID2的激酶结构域（PID2K）发生物理相互作用，并且OsPUB15的ARM重复结构域对这种相互作用至关重要。体外生化实验表明PID2K具有激酶活性，能够磷酸化OsPUB15。我们还发现磷酸化形式的OsPUB15具有E3连接酶活性。表达模式分析表明OsPUB15是组成型表达的，其编码蛋白OsPUB15定位于细胞质中。过表达gospub15的转基因水稻在早期表现出自发的细胞死亡损伤，这与植物基础防御反应的组成性激活有关，包括过氧化氢的过度积累、致病相关基因的上调表达和对稻瘟病菌株的抗性增强。我们还观察到，随着植物的生长，细胞死亡损伤在整个幼苗上迅速蔓延，导致幼苗致死表型。结论E3连接酶OsPUB15可直接与受体样激酶PID2相互作用，调控植物细胞死亡和稻瘟病抗性。
BackgroundRice blast disease is one of the most destructive diseases of rice worldwide. We previously cloned the rice blast resistance genePid2, which encodes a transmembrane receptor-like kinase containing an extracellular B-lectin domain and an intracellular serine/threonine kinase domain. However, little is known aboutPid2-mediated signaling.ResultsHere we report the functional characterization of the U-box/ARM repeat protein OsPUB15 as one of the PID2-binding proteins. We found that OsPUB15 physically interacted with the kinase domain of PID2 (PID2K)in vitroandin vivoand the ARM repeat domain of OsPUB15 was essential for the interaction.In vitrobiochemical assays indicated that PID2K possessed kinase activity and was able to phosphorylate OsPUB15. We also found that the phosphorylated form of OsPUB15 possessed E3 ligase activity. Expression pattern analyses revealed thatOsPUB15was constitutively expressed and its encoded protein OsPUB15 was localized in cytosol. Transgenic rice plants over-expressingOsPUB15at early stage displayed cell death lesions spontaneously in association with a constitutive activation of plant basal defense responses, including excessive accumulation of hydrogen peroxide, up-regulated expression of pathogenesis-related genes and enhanced resistance to blast strains. We also observed that, along with plant growth, the cell death lesions kept spreading over the whole seedlings quickly resulting in a seedling lethal phenotype.ConclusionsThese results reveal that the E3 ligase OsPUB15 interacts directly with the receptor-like kinase PID2 and regulates plant cell death and blast disease resistance.
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影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
影响因子：
7.4
作者：
Cho, Seok Keun;Chung, Hoo Sun;Kim, Woo Taek
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影响因子：
3.9
作者：
Downes, B;Vierstra, RD
通讯作者：
Vierstra, RD
影响因子：
6.8
作者：
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Kuriyan J
影响因子：
3.5
作者：
Guais, Adeline;Siegrist, Sylvie;Bulle, Frederique
通讯作者：
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