Carbon-dependent control of electron transfer and central carbon pathway genes for methane biosynthesis in the Archaean, Methanosarcina acetivorans strain C2A.
Carbon-dependent control of electron transfer and central carbon pathway genes for methane biosynthesis in the Archaean, Methanosarcina acetivorans strain C2A.
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DOI：
10.1186/1471-2180-10-62
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发表时间：
2010-02-23
期刊：
影响因子：
4.2
通讯作者：
Gunsalus RP
中科院分区：
文献类型：
--
作者：
Rohlin L;Gunsalus RP
古细菌，Methanosarcina acetivorans菌株C2 A从各种一碳底物和乙酸盐形成甲烷，一种有效的温室气体。虽然导致甲烷形成的生物化学途径是众所周知的，但对编码碳流、电子转移和/或能量守恒所需蛋白质的许多基因的表达知之甚少。对M中包含100多个基因的20个基因簇进行了定量转录分析。乙酰基食腐菌，其编码在底物转化为甲烷中具有已知或潜在作用的酶/蛋白质。许多看似“冗余”的基因/基因簇的表达建立了约70个基因的底物依赖性控制，用于通过甲醇和乙酸盐利用的途径产生甲烷。这些包括可溶型和膜型异二硫键还原酶（hdr）的基因，氢化酶，包括vht型F420非还原性氢化酶的基因，氘型（fmd）以及钨型（fwd）甲酰基甲呋喃脱氢酶，rnf和mrp型电子转移复合物的基因，乙酸盐摄取的基因，加上aha和atp型ATP合成复合物的多个基因。对7个基因簇的启动子的分析揭示了长度为51-137个核苷酸的UTR前导序列，提高了转录和翻译水平控制的可能性。以上结果证实了两个主要基因家族在M. acetivorans在应对碳/能源供应。此外，定量mRNA测量证明了调节转录本丰度的动态范围。由于M.食乙酸菌也存在于其它甲烷八叠球菌属物种中，包括甲烷八叠球菌。mazei和M. barkeri，这些发现为预测这些环境重要的产甲烷菌的相关控制提供了基础。
The archaeon, Methanosarcina acetivorans strain C2A forms methane, a potent greenhouse gas, from a variety of one-carbon substrates and acetate. Whereas the biochemical pathways leading to methane formation are well understood, little is known about the expression of the many of the genes that encode proteins needed for carbon flow, electron transfer and/or energy conservation. Quantitative transcript analysis was performed on twenty gene clusters encompassing over one hundred genes in M. acetivorans that encode enzymes/proteins with known or potential roles in substrate conversion to methane. The expression of many seemingly "redundant" genes/gene clusters establish substrate dependent control of approximately seventy genes for methane production by the pathways for methanol and acetate utilization. These include genes for soluble-type and membrane-type heterodisulfide reductases (hdr), hydrogenases including genes for a vht-type F420 non-reducing hydrogenase, molybdenum-type (fmd) as well as tungsten-type (fwd) formylmethanofuran dehydrogenases, genes for rnf and mrp-type electron transfer complexes, for acetate uptake, plus multiple genes for aha- and atp-type ATP synthesis complexes. Analysis of promoters for seven gene clusters reveal UTR leaders of 51-137 nucleotides in length, raising the possibility of both transcriptional and translational levels of control. The above findings establish the differential and coordinated expression of two major gene families in M. acetivorans in response to carbon/energy supply. Furthermore, the quantitative mRNA measurements demonstrate the dynamic range for modulating transcript abundance. Since many of these gene clusters in M. acetivorans are also present in other Methanosarcina species including M. mazei, and in M. barkeri, these findings provide a basis for predicting related control in these environmentally significant methanogens.
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影响因子：
3.2
作者：
Schut, GJ;Brehm, SD;Adams, MWW
通讯作者：
Adams, MWW
影响因子：
2.1
作者：
Saum, Regina;Schlegel, Katharina;Mueller, Volker
通讯作者：
Mueller, Volker
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
2.8
作者：
Hochheimer, A;Hedderich, R;Thauer, RK
通讯作者：
Thauer, RK
DOI：
10.1111/j.1432-1033.1997.00226.x
发表时间：
1997-02-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Kunkel, A;Vaupel, M;Hedderich, R
通讯作者：
Hedderich, R