De novo assembly and functional annotation of the olive (Olea europaea) transcriptome.
De novo assembly and functional annotation of the olive (Olea europaea) transcriptome.
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DOI：
10.1093/dnares/dss036
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发表时间：
2013-02
期刊：
影响因子：
--
通讯作者：
Beuzón CR
中科院分区：
文献类型：
--
作者：
Muñoz-Mérida A;González-Plaza JJ;Cañada A;Blanco AM;García-López Mdel C;Rodríguez JM;Pedrola L;Sicardo MD;Hernández ML;De la Rosa R;Belaj A;Gil-Borja M;Luque F;Martínez-Rivas JM;Pisano DG;Trelles O;Valpuesta V;Beuzón CR
橄榄育种计划的重点是选择幼年期短、适合机械收获的植物结构或油脂特性，包括脂肪酸组成、酚类和挥发性化合物，以适应新的市场。了解这些特征的分子基础并提高此类育种计划的效率需要开发基因组信息和工具。然而，尽管有经济上的相关性，关于橄榄或近亲物种的基因组信息仍然很少。我们应用Sanger和454焦磷酸测序技术，从Picual、Arbequina和Lechin de Sevilla栽培品种和Picual×Arbequina杂交后代的分离后代的12个cDNA文库中获得了近200万个读数。文库包括三个相关发育阶段的果实中果皮和种子，幼茎和幼叶，活跃的幼芽和成芽以及休眠的芽，以及幼根和成根。用文库或组织组装读物，然后组装成81020个单基因，平均大小为496个碱基。在这里，我们报告它们的组装和它们的功能注释。
Olive breeding programmes are focused on selecting for traits as short juvenile period, plant architecture suited for mechanical harvest, or oil characteristics, including fatty acid composition, phenolic, and volatile compounds to suit new markets. Understanding the molecular basis of these characteristics and improving the efficiency of such breeding programmes require the development of genomic information and tools. However, despite its economic relevance, genomic information on olive or closely related species is still scarce. We have applied Sanger and 454 pyrosequencing technologies to generate close to 2 million reads from 12 cDNA libraries obtained from the Picual, Arbequina, and Lechin de Sevilla cultivars and seedlings from a segregating progeny of a Picual × Arbequina cross. The libraries include fruit mesocarp and seeds at three relevant developmental stages, young stems and leaves, active juvenile and adult buds as well as dormant buds, and juvenile and adult roots. The reads were assembled by library or tissue and then assembled together into 81 020 unigenes with an average size of 496 bases. Here, we report their assembly and their functional annotation.
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影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick
影响因子：
4.1
作者：
Leon, Lorenzo;De la Rosa, Raul;Rallo, Luis
通讯作者：
Rallo, Luis
影响因子：
5.4
作者：
de la Rosa, R;Angiolillo, A;Baldoni, L
通讯作者：
Baldoni, L
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.7
作者：
Donaire L;Pedrola L;Rosa Rde L;Llave C
通讯作者：
Llave C