Transcription Factor MaMsn2 Regulates Conidiation Pattern Shift under the Control of MaH1 through Homeobox Domain in Metarhizium acridum.
Transcription Factor MaMsn2 Regulates Conidiation Pattern Shift under the Control of MaH1 through Homeobox Domain in Metarhizium acridum.
复制标题
转录因子MaMsn2在MaH1调控下通过同源异型框结构域调控绿僵菌产孢模式转换
DOI：
10.3390/jof7100840
复制
发表时间：
2021-10-07
期刊：
影响因子：
--
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Song D;Cao Y;Xia Y
丝状真菌的生长模式可以根据环境条件在菌丝径向极性生长和非极性酵母样细胞生长之间切换。径向极生长后的无性分生孢子称为正常分生孢子（NC），而酵母样细胞生长称为微循环分生孢子（MC）。先前的研究发现绿僵菌中 MaH1 的破坏导致分生孢子从 NC 转变为 MC。但监管机制尚不明确。在这里，我们发现 MaMsn2（M. acridum 中的 Msn2 同源基因）在 MaH1 被破坏时大幅下调（ΔMaH1）。 MaMsn2 的缺失还导致在营养丰富的培养基上分生孢子从 NC 转变为 MC。酵母单杂交（Y1H）和电泳迁移率变动分析（EMSA）表明MaH1可以结合到MaMsn2基因的启动子区域。破坏MaH1和MaMsn2启动子区之间的相互作用显着下调MaMsn2的转录水平，并且MaMsn2在ΔMaH1中的过表达可以从ΔMaH1的MC恢复NC。我们的研究结果表明，MaMsn2 在 MaH1 直接控制下发挥了维持 NC 模式的作用，这首次揭示了丝状真菌中调节分生孢子模式转变的分子机制。
The growth pattern of filamentous fungi can switch between hyphal radial polar growth and non-polar yeast-like cell growth depending on the environmental conditions. Asexual conidiation after radial polar growth is called normal conidiation (NC), while yeast-like cell growth is called microcycle conidiation (MC). Previous research found that the disruption of MaH1 in Metarhizium acridum led to a conidiation shift from NC to MC. However, the regulation mechanism is not clear. Here, we found MaMsn2, an Msn2 homologous gene in M. acridum, was greatly downregulated when MaH1 was disrupted (ΔMaH1). Loss of MaMsn2 also caused a conidiation shift from NC to MC on a nutrient-rich medium. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assay (EMSA) showed that MaH1 could bind to the promoter region of the MaMsn2 gene. Disrupting the interaction between MaH1 and the promoter region of MaMsn2 significantly downregulated the transcription level of MaMsn2, and the overexpression of MaMsn2 in ΔMaH1 could restore NC from MC of ΔMaH1. Our findings demonstrated that MaMsn2 played a role in maintaining the NC pattern directly under the control of MaH1, which revealed the molecular mechanisms that regulated the conidiation pattern shift in filamentous fungi for the first time.
登录
查看更多内容
影响因子：
4.2
作者：
Cary JW;Harris-Coward P;Scharfenstein L;Mack BM;Chang PK;Wei Q;Lebar M;Carter-Wientjes C;Majumdar R;Mitra C;Banerjee S;Chanda A
通讯作者：
Chanda A
影响因子：
4.5
作者：
Kim S;Park SY;Kim KS;Rho HS;Chi MH;Choi J;Park J;Kong S;Park J;Goh J;Lee YH
通讯作者：
Lee YH
影响因子：
3.3
作者：
Hirata, Y;Andoh, T;Kikuchi, A
通讯作者：
Kikuchi, A
影响因子：
64.5
作者：
ADAMS, TH;BOYLAN, MT;TIMBERLAKE, WE
通讯作者：
TIMBERLAKE, WE
深入了解两种昆虫病原真菌分生孢子形成、多重应激反应和毒力所需的 Msn2 转录调控
影响因子：
3
作者：
Liu, Qian;Ying, Sheng-Hua;Feng, Ming-Guang
通讯作者：
Feng, Ming-Guang