Superpositioning of deletions promotes growth of Escherichia coli with a reduced genome.
Superpositioning of deletions promotes growth of Escherichia coli with a reduced genome.
复制标题
DOI：
10.1093/dnares/dsn019
复制
发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Mori H
中科院分区：
文献类型：
--
作者：
Mizoguchi H;Sawano Y;Kato J;Mori H
大肠杆菌具有多个基因组区域，消除它们可以通过减少不必要的代谢途径和复杂的调控网络来改善细胞的使用。虽然已经构建了几种基因组减少的菌株，但还没有报道构建的缺失菌株对生长的影响。为了保持稳健的生长和基本代谢途径，使用M9基本培养基检查每种缺失菌株的生长和缺失的组合效应。然后，通过整合不影响生长的缺失，构建了基因组减少1 Mb的新菌株MGF-01。MGF-01在指数期与野生型一样生长，并在野生型进入稳定期后继续生长。MGF-01的最终细胞密度是野生型菌株的1.5倍。使用MGF-01作为生产宿主，实现了L-苏氨酸产量的2.4倍增加。
Escherichia coli has dispensable genome regions and eliminating them may improve cell use by reducing unnecessary metabolic pathways and complex regulatory networks. Although several strains with reduced genomes have already been constructed, there have been no reports of strains constructed with deletions assayed for influence on growth. To retain robust growth and fundamental metabolic pathways, the growth of each deletion strain and combination effects of deletions were checked using M9 minimal medium. Then a new strain, MGF-01, with a 1 Mb reduced genome was constructed by integrating deletions that did not affect growth. MGF-01 grew as well as the wild type in the exponential phase and continued growing after the wild type had entered the stationary phase. The final cell density of MGF-01 was 1.5 times higher than that of the wild-type strain. Using MGF-01 as a production host, a 2.4-fold increase in l-threonine production was achieved.
登录
查看更多内容
影响因子：
3.2
作者：
HIRAGA, S;NIKI, H;JAFFE, A
通讯作者：
JAFFE, A
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
--
影响因子：
3.6
作者：
Hashimoto, M;Ichimura, T;Kato, J
通讯作者：
Kato, J
影响因子：
1.6
作者：
Shibasaki, T;Mori, H;Ozaki, A
通讯作者：
Ozaki, A
影响因子：
9.9
作者：
通讯作者：
--