Cardiovascular Organoids/3D Models Review Series: an Introduction.
Cardiovascular Organoids/3D Models Review Series: an Introduction.
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心血管类器官/3D 模型评论系列：简介。
DOI：
10.1161/circresaha.123.322561
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发表时间：
2023
影响因子：
20.1
通讯作者：
StHilaire,Cynthia
中科院分区：
文献类型：
--
作者：
StHilaire,Cynthia
尽管有大量的研究集中在揭示推动心血管疾病进展的病因学和分子机制上，沿着协调一致的公共卫生努力以减轻环境因素，以及近年来取得的突破性药物发现，心脏病仍然是美国的主要死亡原因。[1]今年是美国心脏协会成立100周年，也是《循环研究》出版的白金纪念日，回顾我们的第一期，我们发现70年前的主题在今天同样重要。第一期的手稿涵盖了研究血管床血管反应性差异的研究，2雌激素在动脉粥样硬化中的影响，3 ATP在心脏收缩中的重要性，4以及我们现在所知道的血管平滑肌细胞的收缩性。浏览这些开创性的研究，令人惊讶的是，使用的模型多种多样-人类，狗，猫和鸡-而没有一项研究使用啮齿动物，体外培养血管细胞的方法尚未建立。虽然大型哺乳动物更全面地再现了人类生物学，但研究远离它们的原因有很多。利用小鼠的研究在20世纪初就开始了，但直到20世纪90年代初，它们才成为主要的体内研究系统;它们的优势部分归功于基因工程的进步，它们繁殖的相对速度，以及更低的成本和更小的足迹来容纳这些较小的哺乳动物。[6]与较大的哺乳动物相比，老鼠的成本仍然较低，更容易饲养和照顾，但它们绝不是廉价的。在许多情况下，例如单基因疾病和年龄相关的血管重塑，小鼠不能自然地重现人类疾病的发病机制和时间进程，7，8这些差异导致从实验室到临床的新疗法的高失败率。9.虽然动物模型确实是有用和必要的工具，但我们如何填补其局限性的空白？答案之一在于我们有能力诱导细胞自组装成三维结构，可以概括人类生物学和生理学，这可以作为一种更便宜，更有效的手段来模拟血管疾病和药物测试等。在本综述系列中，我们涵盖了心血管类器官和3D细胞培养模型领域的知识现状。
Even with the vast amount of research focused on uncovering the etiology and molecular mechanisms that drive the progression of cardiovascular disease, along with concerted public health efforts to mitigate environmental contributors, and the groundbreaking drug discoveries made in recent years, heart disease is still the leading cause of death in the United States. 1 This year marks the Centennial anniversary of the founding of the American Heart Association, as well as the platinum anniversary of the publication of Circulation Research, and looking back to our first issue we see that the topics of 70 years ago are just as relevant today. The manuscripts in that first issue covered studies that investigated the differences in vasoreactivity of the vascular beds, 2 the impact of estrogen in atherosclerosis, 3 the importance of ATP in cardiac contraction, 4 and contractility of what we now know to be vascular smooth muscle cells. 5 Scrolling through these seminal studies, it is striking to see the variety of models utilized—humans, dogs, cats, and chickens—while not a single study used rodents, and the methods to culture vascular cells in vitro had not been established yet. While larger mammals more fully recapitulate human biology there are many reasons why research has moved away from them. Studies utilizing mice began in earnest in the early 1900s, but they did not become the primary in vivo system of study until the early 1990s; their advantages owing in part to the advances in genetic engineering, the relative speed of their reproduction, and lower cost and smaller footprint to house these smaller mammals. 6 Mice are still less costly and easier to house and care for than larger mammals, but they are by no means inexpensive. And in many cases, such as with monogenetic disease and age-related vascular remodeling, mice do not naturally recapitulate the pathogenesis and time course of the human maladies, 7, 8 and these differences contribute to the high failure rate of novel therapies that move from the laboratory to the clinic. 9 While animal models are indeed useful and essential tools, how can we fill in the gaps of their limitations? One answer lies in our ability to coax cells to self-assemble into 3 dimensional structures that can recapitulate human biology and physiology, which can serve as a cheaper and more efficient means for modelling vascular disease and for things like drug testing. In this review series we cover the current state of knowledge in the field of cardiovascular organoids and 3D cell culture models.
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DOI：
10.1161/atvbaha.118.311579
发表时间：
2019-03-01
影响因子：
8.7
作者：
Joolharzadeh, Pouya;St Hilaire, Cynthia
通讯作者：
St Hilaire, Cynthia
通过给予胆固醇喂养的雏鸡雌激素-雄激素来预防冠状动脉粥样硬化
影响因子：
20.1
作者：
J. Stamler;R. Pick;L. Katz
通讯作者：
L. Katz
DOI：
--
发表时间：
2013
期刊：
Missouri medicine
影响因子：
--
作者：
Ericsson,AaronC;Crim,MarcusJ;Franklin,CraigL
通讯作者：
Franklin,CraigL
影响因子：
20.1
作者：
P. Khairallah;W. Mommaerts
通讯作者：
W. Mommaerts
来自肢体静脉的局部姿势性血管舒缩反射
影响因子：
20.1
作者：
P. Gaskell;A. C. Burton
通讯作者：
A. C. Burton