Adipose tissue-derived extracellular fraction characterization: biological and clinical considerations in regenerative medicine.
Adipose tissue-derived extracellular fraction characterization: biological and clinical considerations in regenerative medicine.
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DOI：
10.1186/s13287-018-0956-4
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发表时间：
2018-08-09
影响因子：
7.5
通讯作者：
Picardo M
中科院分区：
文献类型：
--
作者：
Bellei B;Migliano E;Tedesco M;Caputo S;Papaccio F;Lopez G;Picardo M
脂肪组织来源的干细胞被认为是细胞治疗和再生医学领域的一个有前途的来源。除了使用脂肪组织或纯化干细胞进行直接细胞替换外，通过脂肪组织复合物进行细胞间分子交换，大量生物活性分泌因子通过减少组织损伤和刺激内源性修复显示出有益的效果。然而，出于治疗目的，使用分泌组衍生物，如全条件培养基或体外生成的纯化外泌体，可能在细胞制造、储存、产品安全性以及作为现成治疗产品的潜力方面存在相当大的缺点。在这项研究中，我们评估了术中从28名健康供体中分离的液体抽脂液对正常人类黑素细胞、角化细胞和成纤维细胞抗氧化应激、抗衰老、体外增殖和迁移的保护作用。免疫酶定量的几种生长因子和重要的信号分子被用来定义生理脂肪组织分泌组的生物学特征。从抽脂液中分离出的脂肪组织细胞外组分（AT-Ex）显示出显著的皮肤修复潜力。AT-Ex以剂量依赖的方式增强真皮和表皮细胞的增殖，而不促进癌细胞的生长。此外，真皮成纤维细胞的迁移，内源性修复的一个重要现象，被AT-Ex治疗增强。当细胞暴露于外部敌对因素时，AT-Ex对氧化应激损伤有积极影响，并防止成纤维细胞衰老表型，包括与皮肤衰老相关的旁分泌功能。总的来说，我们的研究结果表明，携带体内分泌组生理平衡的自然系统可以改善皮肤伤口愈合和组织修复。这种方法代表了再生医学的创新观点和治疗策略，也可以与自体干细胞移植结合治疗慢性不愈合伤口、稳定型白癜风、严重烧伤和肿瘤后疤痕。本文的在线版本（10.1186/s13287-018-0956-4）包含补充材料，授权用户可使用。
Adipose tissue-derived stem cells are considered to be a promising source in the field of cell therapy and regenerative medicine. In addition to direct cell replacement using adipose tissue or purified stem cells, intercellular molecule exchange by the adipose tissue complex, a vast array of bioactive secretory factors, demonstrated beneficial effects by reducing tissue damage and stimulation of endogenous repair. However, for therapeutic purposes, the use of secretome derivatives, such as full conditioned media or purified exosomes generated in vitro, may present considerable disadvantages for cell manufacturing, storage, product safety, and their potential as a ready-to-go therapeutic product. In this study, the effect of a liquid fraction of lipoaspirates isolated intraoperatively from 28 healthy donors was evaluated for their protective effect against oxidative stress and senescence, proliferation, and migration in vitro on normal human melanocytes, keratinocytes, and fibroblasts. Immunoenzymatic quantification of several growth factors and important signal molecules was used to define the biological profile of physiological adipose tissue secretome. Adipose tissue extracellular fraction (AT-Ex), isolated from lipoaspirate, exhibited significant potential for skin repair. AT-Ex augmented dermal and epidermal cell proliferation in a dose-dependent manner without promoting cancer cell growth. Moreover, migration of dermal fibroblasts, an important phenomenon implicated in endogenous repair, was enhanced by AT-Ex treatment. AT-Ex has a positive impact on oxidative stress damage when cells are exposed to extrinsic hostile factors and prevent a fibroblast senescence phenotype including paracrine functions associated with skin aging. Collectively, our findings propose natural systems carrying the physiological balance of in-vivo produced secretome that could improve cutaneous wound healing and tissue repair. This approach, representing an innovative perspective and therapeutic strategy in regenerative medicine, could also be combined with autologous stem cell grafts to treat chronic nonhealing wounds, stable vitiligo, severe burns, and post-oncological scarring. The online version of this article (10.1186/s13287-018-0956-4) contains supplementary material, which is available to authorized users.
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影响因子：
7.5
作者：
Damania A;Jaiman D;Teotia AK;Kumar A
通讯作者：
Kumar A
影响因子：
7.5
作者：
Lai F;Kakudo N;Morimoto N;Taketani S;Hara T;Ogawa T;Kusumoto K
通讯作者：
Kusumoto K
影响因子：
4
作者：
Gan, Qini;Huang, Jing;Tong, Tanjun
通讯作者：
Tong, Tanjun
DOI：
10.1615/critreveukaryotgeneexpr.2015013843
发表时间：
2015-01-01
影响因子：
1.6
作者：
Hendijani, Fatemeh;Javanmard, Shaghayegh Haghjooy
通讯作者：
Javanmard, Shaghayegh Haghjooy
影响因子：
3.7
作者：
Briganti, Stefania;Flori, Enrica;Picardo, Mauro
通讯作者：
Picardo, Mauro