Novel Anti-Adhesive CMC-PE Hydrogel Significantly Enhanced Morphological and Physiological Recovery after Surgical Decompression in an Animal Model of Entrapment Neuropathy.
Novel Anti-Adhesive CMC-PE Hydrogel Significantly Enhanced Morphological and Physiological Recovery after Surgical Decompression in an Animal Model of Entrapment Neuropathy.
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DOI：
10.1371/journal.pone.0164572
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Hirata H
中科院分区：
文献类型：
--
作者：
Urano H;Iwatsuki K;Yamamoto M;Ohnisi T;Kurimoto S;Endo N;Hirata H
我们开发了一种由羧甲基纤维素钠（CMC）衍生的新型水凝胶，其中磷脂酰乙醇胺（PE）被引入到CMC的羧基中以防止神经周围粘连。这种水凝胶在大鼠模型中显示出良好的抗粘连效果，即使在积极的内神经松解后也是如此。在此，我们证实了水凝胶对神经减压后形态学和生理恢复的影响。采用硅胶管制备慢性坐骨神经压迫大鼠模型。神经减压后1、2、3个月，通过运动传导速度（MCV）、胫前肌湿重和神经形态计量学评估，确认形态学和生理恢复。电生理结果显示，CMC-PE组在1个月时恢复速度明显快于对照组（24.0±3.1 vs. 21.0±2.1 m/s），此后MCV恢复速度明显快于对照组（p < 0.05）。CMC-PE （BW）组与对照组1月龄湿肌质量差异显著（0.148±0.020比0.108±0.019%BW）。在整个试验期间，CMC-PE组的平均湿肌质量不断高于对照组。CMC-PE组在1个月时的轴突面积（24.1±17.3 μm2比12.3±9 μm2）是对照组的2倍，这是由于大直径轴突的比例更高。虽然这种趋势在整个实验期间持续，但两个月后差异减小，三个月时差异无统计学意义。虽然抗粘连剂可以减少神经损伤后的粘连，但其对慢性压迫性神经病手术减压后形态学和生理恢复的影响尚未得到详细研究。本研究表明，新型抗黏附CMC-PE凝胶能促进减压术后神经形态和生理的恢复。
We developed a novel hydrogel derived from sodium carboxymethylcellulose (CMC) in which phosphatidylethanolamine (PE) was introduced into the carboxyl groups of CMC to prevent perineural adhesions. This hydrogel has previously shown excellent anti-adhesive effects even after aggressive internal neurolysis in a rat model. Here, we confirmed the effects of the hydrogel on morphological and physiological recovery after nerve decompression. We prepared a rat model of chronic sciatic nerve compression using silicone tubing. Morphological and physiological recovery was confirmed at one, two, and three months after nerve decompression by assessing motor conduction velocity (MCV), the wet weight of the tibialis anterior muscle and morphometric evaluations of nerves. Electrophysiology showed significantly quicker recovery in the CMC-PE group than in the control group (24.0 ± 3.1 vs. 21.0± 2.1 m/s (p < 0.05) at one months and MCV continued to be significantly faster thereafter. Wet muscle weight at one month significantly differed between the CMC-PE (BW) and control groups (0.148 ± 0.020 vs. 0.108 ± 0.019%BW). The mean wet muscle weight was constantly higher in the CMC-PE group than in the control group throughout the experimental period. The axon area at one month was twice as large in the CMC-PE group compared with the control group (24.1 ± 17.3 vs. 12.3 ± 9 μm2) due to the higher ratio of axons with a larger diameter. Although the trend continued throughout the experimental period, the difference decreased after two months and was not statistically significant at three months. Although anti-adhesives can reduce adhesion after nerve injury, their effects on morphological and physiological recovery after surgical decompression of chronic entrapment neuropathy have not been investigated in detail. The present study showed that the new anti-adhesive CMC-PE gel can accelerate morphological and physiological recovery of nerves after decompression surgery.
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影响因子：
2.8
作者：
Yamamoto, Michiro;Endo, Nobuyuki;Hirata, Hitoshi
通讯作者：
Hirata, Hitoshi
影响因子：
3
作者：
ABITBOL, JJ;LINCOLN, TL;GARFIN, SR
通讯作者：
GARFIN, SR
DOI：
10.1142/s0218810402000911
发表时间：
2002-07-01
期刊：
Hand surgery : an international journal devoted to hand and upper limb surgery and related research : journal of the Asia-Pacific Federation of Societies for Surgery of the Hand
影响因子：
--
作者：
Ikeda, Kazuo;Yamauchi, Daisuke;Tomita, Katsuro
通讯作者：
Tomita, Katsuro
影响因子：
4.8
作者：
Dam-Hieu, Phong;Lacroix, Catherine;Tadie, Marc
通讯作者：
Tadie, Marc
影响因子：
11
作者：
SUNDERLAND, S
通讯作者：
SUNDERLAND, S