In silico optimization of a fragment-based hit yields biologically active, high-efficiency inhibitors for glutamate racemase.
In silico optimization of a fragment-based hit yields biologically active, high-efficiency inhibitors for glutamate racemase.
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DOI：
10.1002/cmdc.201300271
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发表时间：
2013-10
期刊：
影响因子：
3.4
通讯作者：
Spies, M. Ashley
中科院分区：
文献类型：
--
作者：
Whalen, Katie L.;Chau, Anthony C.;Spies, M. Ashley
A novel lead compound for inhibition of the antibacterial drug target, glutamate racemase, is optimized for both ligand efficiency and lipophilic efficiency. A previously developed hybrid MD-docking and scoring scheme, FERM-SMD, is utilized to predict relative potencies of potential derivatives prior to chemical synthesis. This scheme was successful in distinguishing between high and low affinity binders with minimal experimental structural information, saving time and resources in the process. In vitro potency is increased approximately 4-fold against glutamate racemase from the model organism, B. subtilis. Lead derivatives show 2- to 4-fold increased antimicrobial potency over the parent scaffold. In addition, specificity toward B. subtilis, over E. coli and S. aureus, show dependency on the chemical substituent added to the parent scaffold. Finally, insight is gained into the capacity for these compounds to reach the target enzyme in vivo using a bacterial cell wall lysis assay. The result of this study is a novel small molecule inhibitor of GR with the following characteristics: Ki = 2.5 μM, LE = 0.45 kcal/mol/atom, LiPE = 6.0, MIC50 = 260 μg/mL against B. subtilis, EC50,lysis = 520 μg/mL against B. subtilis
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