Comparing anesthetic stations and environmental DNA sampling to determine community composition of cryptobenthic coral reef fishes of Vava’u, Kingdom of Tonga
Comparing anesthetic stations and environmental DNA sampling to determine community composition of cryptobenthic coral reef fishes of Vava’u, Kingdom of Tonga
复制标题
比较麻醉站和环境 DNA 采样以确定汤加王国瓦瓦乌隐底栖珊瑚礁鱼类的群落组成
DOI：
10.1007/s00338-023-02375-7
复制
发表时间：
2023
期刊：
影响因子：
3.5
通讯作者：
Tornabene, Luke M.
中科院分区：
文献类型：
--
作者：
Gómez-Buckley, Marta C.;Gallego, Ramón;Arranz, Vanessa;Halafihi, Tuikolongahau;Stone, Karen;Erdmann, Mark;Tornabene, Luke M.
隐底礁鱼（CRF）是珊瑚礁群落的重要组成部分，往往被忽视，因为它们的神秘性和非常小的尺寸使它们难以使用视觉方法进行评估。量化CRF群落的流行方法是使用围栏和麻醉剂从珊瑚礁内收集鱼类。环境DNA（eDNA）的珊瑚礁周围的水采样可能是一种替代或补充的方式来量化CRF社区。在这里，我们比较的能力，CRF社区使用从珊瑚礁的间隙内收集的水样中的cDNA序列的特征，从相同的微生境相应的麻醉站。从麻醉站共收集了来自33个物种的676个CRF，而从相同地点采集的eDNA样品中仅检测到4种CRF。导致这些结果的主要因素可能是：（1）CRF的低现存生物量，因此在eDNA水样中可检测的DNA丰度低;（2）大量的非靶向后生动物读段“淹没”了特定CRF序列的检测;以及（3）公共数据库中缺乏代表CRF中存在的高水平特有性的序列，需要更完整的分类学库存
Cryptobenthic reef fishes (CRF) are an important component of coral reef communities that are often overlooked, as their cryptic nature and very small size make them difficult to assess using visual methods. The prevailing method for quantifying communities of CRF is to use enclosures and anesthetics to collect fishes from within the reef. Environmental DNA (eDNA) sampling of water around reefs may be an alternative or supplemental way to quantify CRF communities. Here, we compare the ability to characterize CRF communities using eDNA sequences from water samples collected from within the interstices of the reef, with corresponding anesthetic stations from the same microhabitat. A total of 676 CRF from 33 species were collected from anesthetic stations, whereas only four species of CRF were detected from the eDNA samples taken at the same locations. Main factors contributing to these results may have been: (1) low standing biomass of CRF, thus low abundance of detectable DNA in the eDNA water samples; (2) large number of non-targeted metazoan reads that “drowned” the detections of specific CRF sequences; and (3) lack of sequences available in public databases that represent the high level of endemism present in CRF and a need for more complete taxonomic inventory.
登录
查看更多内容
影响因子：
1.3
作者：
Tornabene L;Greenfield DW;Erdmann MV
通讯作者：
Erdmann MV
影响因子：
3.4
作者：
Lin HC;Hastings PA
通讯作者：
Hastings PA
影响因子：
4.6
作者：
Kelly, Ryan P.;Shelton, Andrew Olaf;Gallego, Ramon
通讯作者：
Gallego, Ramon
神秘物种的聚宝盆 - 戈壁鱼属 Trimma（Percomorpha、Gobiiformes）的 DNA 条形码分析
影响因子：
1.3
作者：
R. Winterbottom;R. Hanner;M. Burridge;Margaret Zur
通讯作者：
Margaret Zur
影响因子：
3.7
作者：
Plaisance L;Caley MJ;Brainard RE;Knowlton N
通讯作者：
Knowlton N