Chimeric coronavirus-like particles carrying severe acute respiratory syndrome coronavirus (SCoV) S protein protect mice against challenge with SCoV.
Chimeric coronavirus-like particles carrying severe acute respiratory syndrome coronavirus (SCoV) S protein protect mice against challenge with SCoV.
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DOI：
10.1016/j.vaccine.2007.11.092
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发表时间：
2008-02-06
期刊：
影响因子：
5.5
通讯作者：
Makino S
中科院分区：
文献类型：
--
作者：
Lokugamage KG;Yoshikawa-Iwata N;Ito N;Watts DM;Wyde PR;Wang N;Newman P;Kent Tseng CT;Peters CJ;Makino S
我们测试了冠状病毒样颗粒（VLP）保护小鼠免受严重急性呼吸综合征冠状病毒（SCoV）感染的功效。将SCoV S蛋白与小鼠肝炎病毒E、M、N蛋白在293 T或CHO细胞中共表达，获得了携带SCoV S蛋白的嵌合VLP。以四周的间隔用嵌合VLP和明矾的混合物接种两次的Balb/c小鼠被保护免于SCoV攻击，如肺中不存在感染性病毒所指示的。相同组的小鼠具有高水平的SCoV特异性中和抗体，而阴性对照组中未用嵌合VLP免疫的小鼠未能表现出中和抗体，表明SCoV特异性中和抗体对于抑制肺内的病毒复制是重要的。尽管炎性浸润的细胞组成存在一些差异，但与阴性对照小鼠相比，我们在嵌合体-VLP处理的小鼠中未观察到任何明显的肺病理学。我们的研究结果表明，嵌合VLP可以是一种有效的疫苗策略，针对SCoV感染。
We tested the efficacy of coronavirus-like particles (VLPs) for protecting mice against severe acute respiratory syndrome coronavirus (SCoV) infection. Coexpression of SCoV S protein and E, M and N proteins of mouse hepatitis virus in 293T or CHO cells resulted in the efficient production of chimeric VLPs carrying SCoV S protein. Balb/c mice inoculated with a mixture of chimeric VLPs and alum twice at an interval of four weeks were protected from SCoV challenge, as indicated by the absence of infectious virus in the lungs. The same groups of mice had high levels of SCoV-specific neutralizing antibodies, while mice in the negative control groups, which were not immunized with chimeric VLPs, failed to manifest neutralizing antibodies, suggesting that SCoV-specific neutralizing antibodies are important for the suppression of viral replication within the lungs. Despite some differences in the cellular composition of inflammatory infiltrates, we did not observe any overt lung pathology in the chimeric-VLP-treated mice, when compared to the negative control mice. Our results show that chimeric VLP can be an effective vaccine strategy against SCoV infection.
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