Imaging type-III secretion reveals dynamics and spatial segregation of Salmonella effectors.
Imaging type-III secretion reveals dynamics and spatial segregation of Salmonella effectors.
复制标题
DOI：
10.1038/nmeth.1437
复制
发表时间：
2010-04
期刊：
影响因子：
48
通讯作者：
Palmer, Amy E.
中科院分区：
文献类型：
--
作者：
Van Engelenburg, Schuyler B.;Palmer, Amy E.
III型分泌系统（T3SS）使革兰氏阴性细菌能够将效应蛋白注入真核宿主细胞。一旦进入，T3SS效应器合作重新编程宿主细胞，使细菌存活。了解效应器在宿主细胞内何时何地定位的进展一直受到缺乏在天然细胞环境中研究这些蛋白质的工具的阻碍。我们报告了一种方法来标记和跟踪T3SS效应在感染过程中使用分裂GFP系统。通过标记来自沙门氏菌的三种效应物（PipB2、SteA和SteC）并表征其在宿主细胞内的定位来证明该技术的广度。PipB2显示出高度动态的行为，从沙门氏菌发出的小管含有空泡标记的内和外标记。SteA优先富集在定位于高尔基体标记的小管上。这种分离表明效应子靶向和定位可能在感染过程中发挥功能性作用。
The type-III secretion system (T3SS) enables gram-negative bacteria to inject effector proteins into eukaryotic host cells. Upon entry, T3SS effectors work cooperatively to reprogram host cells, enabling bacterial survival. Progress in understanding when and where effectors localize within host cells has been hindered by a dearth of tools to study these proteins in the native cellular environment. We report a method to label and track T3SS effectors during infection using a split-GFP system. The breadth of this technique is demonstrated by labeling three effectors from Salmonella (PipB2, SteA, and SteC) and characterizing their localizations within host cells. PipB2 displays highly dynamic behavior on tubules emanating from the Salmonella containing vacuole labeled with both endo- and exocytic markers. SteA is preferentially enriched on tubules localizing with Golgi markers. This segregation suggests effector targeting and localization may play a functional role during infection.
登录
查看更多内容
影响因子：
3.1
作者：
Garcia, Julie Torruellas;Ferracci, Franco;Plano, Gregory V.
通讯作者：
Plano, Gregory V.
影响因子：
4.8
作者：
Boucrot, E;Beuzón, CR;Méresse, S
通讯作者：
Méresse, S
DOI：
10.1073/pnas.0503407102
发表时间：
2005-08-30
影响因子：
11.1
作者：
Schlumberger, MC;Müller, AJ;Hardt, WD
通讯作者：
Hardt, WD
影响因子：
--
作者：
VanEngelenburg, Schuyler B.;Palmer, Amy E.
通讯作者：
Palmer, Amy E.
影响因子：
3.4
作者：
Bakowski, Malina A.;Cirulis, Judith T.;Brumell, John H.
通讯作者：
Brumell, John H.