Amperometric measurements at cells support a role for dynamin in the dilation of the fusion pore during exocytosis.
Amperometric measurements at cells support a role for dynamin in the dilation of the fusion pore during exocytosis.
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DOI：
10.1002/cphc.201300319
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发表时间：
2013-07-22
期刊：
影响因子：
--
通讯作者：
Ewing AG
中科院分区：
文献类型：
--
作者：
Trouillon R;Ewing AG
Dynamin是一种GTPase机械力化学酶，参与内吞作用的后期步骤，将内吞小泡从细胞膜中分离出来。然而，最近的报道强调了它在胞吐中的作用。在这种情况下，动力素可能有助于控制胞吐毛孔，从而表明直接控制神经递质的外流。动力蛋白GTP酶活性的选择性抑制剂dynasore被用来研究动力蛋白在胞吐中的作用。用安培法分析胞吐作用，从而揭示了SARIGORE以剂量依赖的方式抑制胞吐。胞吐峰分析表明，动力蛋白GTPase活性的抑制导致了更短、更小的事件。这一观察结果，加上王朝的快速作用，表明GTP酶的封闭导致了更窄和更短暂的融合孔的形成。这些结果表明，Dynamin的GTPase特性与胞吐释放的持续时间和动力学有关。有趣的是，与其在内吞作用中的作用形成强烈对比的是，动力素的机械力化学性质似乎有助于胞吐过程中毛孔的扩张和稳定。
Dynamin is a GTPase mechanochemical enzyme involved in the late steps of endocytosis, where it separates the endocytotic vesicle from the cell membrane. However, recent reports have emphasized its role in exocytosis. In this case, dynamin may contribute to the control of the exocytotic pore, thus suggesting a direct control on the efflux of neurotransmitters. Dynasore, a selective inhibitor of the GTPase activity of dynamin, was used to investigate the role of dynamin in exocytosis. Exocytosis was analyzed by amperometry, thus revealing that dynasore inhibits exocytosis in a dose-dependent manner. Analysis of the exocytotic peaks showed that the inhibition of the GTPase activity of dynamin led to shorter, smaller events. This observation, together with the rapid effect of dynasore, suggests that the blocking of the GTPase induces the formation of a more narrow and short-lived fusion pore. These results suggest that the GTPase properties of dynamin are involved in the duration and kinetics of exocytotic release. Interestingly, and in strong contrast with its role in endocytosis, the mechanochemical properties of dynamin appear to contribute to the dilation and stability of the pore during exocytosis.
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DOI：
10.1083/jcb.81.2.275
发表时间：
1979-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heuser JE;Reese TS;Dennis MJ;Jan Y;Jan L;Evans L
通讯作者：
Evans L
影响因子：
11.8
作者：
Macia, Eric;Ehrlich, Marcelo;Kirchhausen, Tomas
通讯作者：
Kirchhausen, Tomas
影响因子：
3.9
作者：
Graham, ME;Fisher, RJ;Burgoyne, RD
通讯作者：
Burgoyne, RD
影响因子：
7.4
作者：
Adams, Kelly L.;Engelbrektsson, Johan;Voinova, Marina;Zhang, Bo;Eves, Daniel J.;Karlsson, Roger;Heien, Michael L.;Cans, Ann-Sofie;Ewing, Andrew G.
通讯作者：
Ewing, Andrew G.
影响因子：
4.5
作者：
Fisher, RJ;Burgoyne, RD
通讯作者：
Burgoyne, RD